2型糖尿病肾病亚甲基四氢叶酸还原酶基因多态性研究

目的探讨亚甲基四氢叶酸还原酶(methylenetetrahydrofolate

An association study of methylenetetrahydrofolate reductase gene polymorphism with diabetic nephropathy

Objective:Diabetic vascular diseases and other complications are associated with homocysteinemia in which methylenetetrahydromfolate reductase(MTHFR)gene mutation is involved.In this study,we investigated the association between MTHFR C677T polymorphism and diabetic nephropathy in Chinese patients. Methodology:In:this study 206 cases of type 2 diabetes mellitus(among them 112 developed diabetic nephropathy,and 76 developed diabetic retinopathy)were included and 57 as healthy controls.Genetic polymorphism of MTHFR C677T was determined by PCR RFLP. Results:Increased frequencies of TT genotype and T allele in type 2 diabetes mellitus patients with diabetic nephropathy were found comparing to those without diabetic nephropathy(25 0% vs 19 1%, P <0 05;49 6% vs 37 8%, P <0 05)and the normal control (25 0% vs 17 5%, P <0 01;49 6% vs 31 6%, P <0 01).No significant difference was shown in TT genotype or T allele frequency between the type 2 diabetes mellitus patients without diabetic nephropathy and the controls.Frequencies of TT genotype and T allele were also found in type 2 diabetes mellitus patients with retinopathy as comparing to those without diabetic retinopathy(25 0% vs 20 8%, P <0 05;51 3% vs 40 0%, P <0 05)and normal controls(25 0% vs 17 5%, P <0 01;51 3% vs 31 6%, P <0 01). Conclusion:The genetic polymorphism of MTHFR C677T is associated with the development of diabetic nephropathy and diabetic retinopathy.The T allele may be a risk factor for the development of vascular complications in type 2 diabetes mellitus.