自建检测系统测定血清总蛋白测量不确定度的评估 |
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引用本文: | 吕赛平,刘琴,王春阳,金国兵,邹学森. 自建检测系统测定血清总蛋白测量不确定度的评估[J]. 实验与检验医学, 2014, 0(2): 137-138,149 |
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作者姓名: | 吕赛平 刘琴 王春阳 金国兵 邹学森 |
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作者单位: | 吕赛平 (江西省肿瘤医院检验科,江西 南昌,330029); 刘琴 (江西省肿瘤医院检验科,江西 南昌,330029); 王春阳 (江西省肿瘤医院检验科,江西 南昌,330029); 金国兵 (江西省肿瘤医院检验科,江西 南昌,330029); 邹学森 (江西省肿瘤医院检验科,江西 南昌,330029); |
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摘 要: | 目的评估我科白建检测系统测定血清总蛋白的测量不确定度。方法根据血清总蛋白的医学决定水平(MDL)配制两个浓度水平的试验样本.分别定义为低值MDL样本、高值MDL样本。先评估血清总蛋白在低值MDL及高值MDL的批内重复性、批间重复性、方法偏倚等因子,再根据上述因子确定血清总蛋白在上述两个MDL水平处的扩展不确定度(EU)。结果(1)自建检测系统测定血清总蛋白在低值MDL的批内变异系数(CVw)、批间变异系数(CVB)、方法偏倚变异系数(CVBiaw)分别为0.86%、1.37%、2.31%,EU为5.64%。(2)自建检测系统测定血清总蛋白在高值MDL的CVW、CVB、CVBias。分别为0.74%、1.41%、3.29%,EU为7.31%。结论本研究评估测量不确定度的方法简单、易行,试验所得的EU可以准确地反映临床检验结果的分散性。
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关 键 词: | 检测系统 总蛋白 测量不确定度 医学决定水平 变异系数 |
Evaluation of measurement uncertainty of serum total protein detected by serf-developed detecting system |
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Affiliation: | Lue Saiping,LlU Qin, WANG Chunyang, et al. Department of Laboratory Medicine, Jiangxi Provincial Tumor Hospital, Nanchang 330029, China |
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Abstract: | Objective To evaluate the measurement uncertainty of serum total protein detected by the self-developed detecting system in our department. Methods Two groups of samples were prepared according to medical decision levels(MDL) of serum total protein, which were defined as low-value MDL sample and high-value MDL sample respectively. The within-run repeatability, between-run repeatability and method biases of serum total protein at the 2 levels of MDLs were evaluated firstly, and then the corresponding expanded uncertainties (EU) were confirmed. Results (1)The within-run coefficient of variation(CVw), between-run coefficient of variation(CVB) and methods bias coefficient of variation(CVBias) of serum total protein at the low-value MDL were 0.86%, 1.37% and 2.31% respectively, and the EU was 5.64%. (2)The CVw , CVB and CVBias of serum total protein at the high-value MDL were 0.74%, 1.41% and 3.29% respectively, and the EU was 7.31%. Conclusion This method for evaluating measurement uncertainty in this study is of convenience and feasibility, and the EU can reflect the dispersion of clinical test results accurately. |
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Keywords: | Detecting system Total protein Measurement uncertainty Medical decision level Coefficient of variation |
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