胃癌患者血清可溶性B7 H4的检测及其临床意义

目的检测胃癌患者血清中可溶性B7 H4（sB7 H4）的水平并探讨其临床意义。方法应用酶联免疫吸附试验（ELISA）夹心法检测61例胃癌患者与51例正常人血清sB7 H4水平，分析其与病理类型、治疗前后、胃癌其他血清标志物的相关性。结果胃癌患者血清sB7 H4水平（49.14±16.16）μg/L明显高于正常人（32.67±12.28）μg/L，P＜0.01。低分化腺癌患者sB7 H4水平（64.51±20.67）μg/L高于高分化腺癌（36.78±13.39）μg/L、粘液腺癌（42.79±12.31）μg/L和印戒细胞癌（44.62±12.27）μg/L，P＜0.05。手术前胃癌患者血清sB7 H4水平（58.09±15.28）μg/L明显高于术后（38.58±9.49）μg/L，P＜0.01。胃癌患者血清sB7 H4水平与CEA、CA19 9水平呈正相关（P＜0.01）。结论胃癌患者血清中高水平的sB7 H4及其与病理类型、治疗情况、及其他肿瘤标志物相关，提示sB7 H4可能在胃癌的发生发展中有重要作用，可为胃癌的诊断和治疗提供一种新的靶位点。

Expression of soluble B7-H4 in the serum and its clinical significance in gastric cancer

To determine the serum level of soluble B7 H4 in gastric cancer and to explore its clinical significance. MethodUsing the ELISA sandwich method, the serum level of soluble B7 H4 was determined in 61 cases of gastric cancer and 51 cases of normal controls, then correlations between soluble B7 H4 and pathological types, before and after surgical treatment and other serum markers of gastric cancers were analyzed. ResultsThe serum level of soluble B7 H4 in gastric cancer ［（49.14±16.16）μg/L］was significantly higher than that in normal people［（32.67±12.28）μg/L］, and there was a significant difference (P＜0.01). The serum level of soluble B7 H4 in poorly differentiated adenocarcinoma ［(64.51±20.67)μg/L］was higher than that in well differentiated adenocarcinoma［（36.78±13.39）μg/L］, mucinous adenocarcinoma［(42.79±12.31)μg/L］ , and signet ring cell carcinoma［（44.62±12.27）μg/L］, and there were significant differences among them (P＜0.05). The serum level of soluble B7 H4 in gastric cancer before surgical treatment［（58.09±15.28）μg/L］was higher than that in gastric cancer after surgical treatment［（38.58±9.49）μg/L］(P＜0.01). The serum level of sB7 H4 in gastric cancer was positivelycorrelated with serum levels of CEA and CA19 9（P＜0.01）. ConclusionA high level of serum soluble B7 H4 in gastric cancer has a positive relationship to pathological types, surgical treatment and other serum markers of gastric cancers, which reveals that soluble B7 H4 plays an important role in cancer generation and development.