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达玛烷苷元对睡眠干扰所致小鼠学习记忆障碍的改善作用
引用本文:卜兰兰,石哲,武宏伟,卢聪,王克柱,李莹辉,曲丽娜,刘新民. 达玛烷苷元对睡眠干扰所致小鼠学习记忆障碍的改善作用[J]. 中国比较医学杂志, 2014, 24(10): 48-53,66
作者姓名:卜兰兰  石哲  武宏伟  卢聪  王克柱  李莹辉  曲丽娜  刘新民
作者单位:湖南中医药大学 病理学教研室, 湖南, 长沙 410208;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;中国航天员中心航天医学基础与应用国家重点实验室, 北京 100094;中国航天员中心航天医学基础与应用国家重点实验室, 北京 100094;湖南中医药大学 病理学教研室, 湖南, 长沙 410208;湖南省实验动物中心 湖南省药物安全评价研究中心, 湖南 长沙 410331
基金项目:国际科技合作专项-人参益智药效与基因/蛋白表达谱关联规律合作研究(编号:2011DFA32730);全军医学科技“十二五”科研项目(编号:BWS11J052);极限环境认知损伤保护药临床前药效评价技术体系-国家科技重大专项课题(编号:2012ZX09J12201);湖南中医药大学 病理学与病理生理学重点学科(2012)。
摘    要:目的:研究达玛烷苷元(dammaranesapogenins,DS-1226)对睡眠干扰(sleepinterruption,SI)致认知障碍小鼠学习记忆能力的影响。方法将ICR小鼠随机分为对照组,模型组,DS-1226低、中、高三个剂量组。用改良滚筒法对小鼠进行睡眠干扰,同时灌胃给药15d后,用自主活动仪检测自主活动,水迷宫、避暗实验检测学习记忆能力。结果与模型组比较,DS-1226各给药组总路程、平均速度、运动总时间均增加。模型组水迷宫定位航行D5、D6潜伏期增加;与模型组和低剂量组比较,DS-1226中剂量组D6潜伏期降低,高剂量组D1-D6潜伏期降低;与中剂量组比较,DS-1226高剂量组D1-D5潜伏期降低。模型组水迷宫空间探索目标象限穿台次数及游程比率均低于对照组;DS-1226高剂量组目标象限穿台次数、目标象限游程比率及时间比率均高于模型组,目标象限穿台次数高于中剂量组,目标象限游程比率高于低剂量组。模型组避暗学习错误次数、暗室路程、暗室时间、暗室静息时间增加;与模型组比较,DS-1226低、中剂量组错误次数、暗室路程减少,高剂量组错误次数、暗室时间、暗室路程、暗室静息时间减少,明室时间增加。结论水迷宫和避暗实验结果表明DS-1226给药对SI所致小鼠学习记忆障碍有改善作用,且有剂量相关性。

关 键 词:DS-1226  滚筒  睡眠干扰  ICR小鼠  自主活动  水迷宫  避暗
修稿时间:2014-08-24

Improving effects of dammarane sapogenins on sleep interruption-induced learning and memory impairment in mice
BU Lan-lan,SHI Zhe,WU Hong-wei,LU Cong,WANG Ke-zhu,LI Ying-hui,QU Li-na and LIU Xin-min. Improving effects of dammarane sapogenins on sleep interruption-induced learning and memory impairment in mice[J]. Chinese Journal of Comparative Medicine, 2014, 24(10): 48-53,66
Authors:BU Lan-lan  SHI Zhe  WU Hong-wei  LU Cong  WANG Ke-zhu  LI Ying-hui  QU Li-na  LIU Xin-min
Affiliation:Department of Pathology, Hunan University of Chinese Medicine, Changsha 410208, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193;the State Key Lab of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing 100094;the State Key Lab of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beijing 100094;Department of Pathology, Hunan University of Chinese Medicine, Changsha 410208, China;Hunan Laboratory Animal Center, Hunan Provincial Research Center for Safety Evaluation of Drugs, Changsha 410331
Abstract:Objective To study the effects of dammarane sapogenins (DS-1226) on sleep interruption-induced learning and memory impairment in mice. Methods 130 SPF healthy 5-6-week old male ICR mice were randomly divided into control, model, DS-1226 low dose, DS-1226 medium dose and DS-1226 high dose groups. The behavioral alterations in open field (OF), Morris water maze (MWM) and step-through (ST) tests were detected at 15 days after rotating drum-induced sleep interruption (SI). Results The total distance, movement speed, total duration of movement were increased in OF test (P<0.05, vs. the model group) after treatment. The latency of place navigation was increased from day 5 in the MWM test after 15 d sleep interruption, and the number of crossing in the target quadrant and the percent distance in target quadrant were decreased after 15 d sleep interruption (P<0.05, vs. the control group), while the latency of place navigation was decreased, and the percent distance in target quadrant and percent time in target quadrant after high dose DS-1226 oral administration (P<0.05, vs. the model group) were increased. Error times, distance in dark chamber, time in dark chamber and immobility time in dark chamber were increased in training of step through test (P<0.05, vs. the control group); while these indexes were decreased after DS-1226 oral administration (P<0.05, vs. the model group). But there was no significant difference in the step through testing course. Conclusions The results show that orally administrated DS-1226 can ameliorate SI-induced learning and memory impairment, and there is a significant dosage-effect relationship.
Keywords:Dammarane sapogenins  Rotating drum test  Sleep Interruption  ICR mice  Spontanous activity  Morris water maze test  Step-through test
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