减肥中成药中非法添加酚酞的拉曼光谱快速检测

目的:研究采用拉曼光谱法快速检测减肥中成药中非法添加的酚酞。方法:本文首先对4份减肥中成药样品各加入5 mL甲醇,超声10 min进行提取,接下来对提取液进行过滤,最后直接进行拉曼光谱的测试。结果:建立了酚酞-甲醇溶液的标准曲线,并且对添加不同质量浓度酚酞的减肥中成药进行拉曼光谱检测,所得结果与酚酞实际添加量一致。酚酞在0.1%以上浓度时,浓度与拉曼信号强度呈良好的线性关系(r=0.998 1),RSD 3.7%。结论:结合简单的前处理方法,拉曼光谱法可对减肥中成药中非法添加的酚酞进行快速筛查,并可进行定量分析;该方法快速、简便、成本低,检测限达1%,可同时得到定性与定量结果。

Rapid Detection of Adulterated Phenolphthalein to Chinese Pattern Diet Drugs by Raman Spectroscopy

Objective: To study the rapid detection of phenolphthalein content adulterated to Chinese pattern diet drugs by Raman Spectroscopy. Method: In this paper, 4 portions of Chinese pattern diet drugs were extracted by 5 mL of methanol, sonicated for 10 min, then filtered and tested directly by raman spectroscopy. Result: Calibration curve was defined and analysis was performed on samples with different contents of Phenolphthalein. Results were in accordance with real amount of adulteration. The concentration of phenolphthalein and the intensity of its raman signal was in good linear at the concentration above 0.1% (r=0.998 1, RSD 3.7%). Conclusion: Samples adulterated with phenolphthalein, pretreated by a suitable method could be fast screened by raman spectroscopy. This method is fast, simple and low-cost with the detection limit at 1% for phenolphthalein. Either qualitative or quantitative results can be obtained simultaneously.