动态优化方法对维C银翘片中牛蒡子提取时间的优化

目的:采用动态优化方法优选维C银翘片中牛蒡子的提取工艺。方法:采用HPLC测定牛蒡子苷含量,检测波长280 nm,流动相乙腈-水(20∶80)。通过比较动态优化工艺与原提取工艺对维C银翘片提取率、指标成分含量和指纹图谱的相似度,评价动态优化方法应用于维C银翘片中牛蒡子提取工艺优化的可行性。结果:动态优化工艺与原提取工艺所得提取物基本一致。动态优化工艺为加8倍量60%乙醇回流提取2次,提取时间分别为140,60 min;提取时间较原工艺节约58%,耗电量节约53%。结论:动态优化方法可运用于维C银翘片中牛蒡子提取工艺的优化,该工艺能减少提取时间、降低能耗,具有明显的经济效益。

Application of Dynamic Optimization Method for Extraction Time of Arctii Fructus in Vitamin C Yinqiao Pian

Objective: To optimize extraction process of Arctii Fructus in Vitamin C Yinqiao Pian by dynamic optimization extraction method(DOEM). Method: HPLC was employed to determine the content of arctiin with detection wavelength at 280 nm and mobile phase of acetonitrile-water(20:80).Taking yield and content of arctiin,fingerprints similarity as indexes,feasibility of DOEM using in Arctii Fructus from Wei C Yinqiao tablets was evaluated,and compared with extraction process in 2010 edition of . Result: There was no difference in extract between dynamic optimization process and ordinary extraction process.Optimized DOEM was as following:reflux extracted twice with 8 times the amount of 60% ethanol for extracting time of 140 min and 60 min;it could save 58% of extraction time and 53% of power consumption. Conclusion: DOEM can be applied to optimize extraction process of Arctii Fructus in Vitamin C Yinqiao Pian,it can significantly reduce extraction time and production costs.