黄芩不同提取物溶出度和抗氧化性能评价

目的:测定黄芩不同提取物的溶出度并评价其抗氧化性能。方法:根据前处理工艺收集含不同伴生物质的黄芩提取物(黄芩苷质量分数分别为19.75%,62.83%,84.55%,98.0%),采用HPLC测定各提取物中黄芩苷的溶出度,流动相甲醇-水-磷酸(47∶53∶0.2),检测波长280 nm。利用DPPH模型测定不同黄芩提取物抗氧化的能力,检测波长516 nm。结果:不同伴生物质黄芩提取物中黄芩苷含量越高,溶出度降低;4种提取物的3 h累积溶出率分别为38.24%,25.91%,23.49%,22.98%。抗氧化能力随着提取物中黄芩苷含量的增加而提高,当黄芩苷质量分数85%时,抗氧化能力无明显变化。结论:不同质与量的伴生物质对黄芩提取物中黄芩苷的溶出度和抗氧化性能具有一定影响。

Evaluation of Dissolution and Oxidation Resistance of Different Extracts Obtained from Scutellariae Radix

Objective: To determine dissolution of different extracts of Scutellariae Radix and investigate its oxidation resistance. Method: According to pre-treatment process to collect extracts of Scutellariae Radix containing different associated substances(The content of baicalin were 19.75%,62.83%,84.55% and 98.0%),HPLC was employed to determine dissolution of baicalin in each sample with mobile phase of methanol-water-phosphoric acid(47:53:0.2) and detection wavelength at 280 nm.Antioxidant capacity of different extracts of Scutellariae Radix was measured by DPPH,detection wavelength was set at 516 nm. Result: The higher baicalin content in different associated substances,the lower bioavailability;cumulative dissolution rates of these four extracts in 3 h were 38.24%,25.91%,23.49% and 22.98%.With increasing the content of baicalin in extracts,antioxidant capacity increased,there were no obvious changes in antioxidant capacity when the content of baicalin reached above 85%. Conclusion: Different quality and quantity of associated substances have a certain effect on dissolution and oxidation resistance of baicalin.