| 肝癌细胞系HEpG2表达FasL杀伤T淋巴细胞 |
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| 引用本文： | 冯荻,李伟毅,席晔斌,陈广洁,周光炎.肝癌细胞系HEpG2表达FasL杀伤T淋巴细胞[J].现代免疫学,2000,20(5):260-262. |
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| 作者姓名： | 冯荻 李伟毅 席晔斌 陈广洁 周光炎 |
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| 作者单位： | 上海第二医科大学,上海市免疫学研究所,上海,200025 |
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| 摘 要： | 为研究肝肿瘤细胞系表达FasL逃逸免疫监视的可能 ,采用逆转录 PCR方法和免疫组化法测肝癌细胞株FasL的表达 ,并测T淋巴细胞的凋亡。结果发现HEpG2细胞在mRNA水平和蛋白质水平上皆可检测到FasL的高表达 ,而 772 1细胞未能测到。HEpG2细胞与JurkatT淋巴细胞共培养后 ,19 4%的T细胞发生凋亡 ,抗FasL的单抗能阻断这种凋亡。因而肝肿瘤细胞HEpG2表达功能性的FasL ,能杀伤Fas阳性1] 的T淋巴细胞。
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| 关 键 词： | 肝癌细胞 FasL 细胞凋亡 免疫豁免 |
| 文章编号： | 1001-2478(2000)05-0260-03 |
| 修稿时间： | 1999-09-27 |
Fas Ligand Expressed on HEpG2 Cells Induces the Cytotoxicity of T Lymphocytes |
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| Feng Di,Li Weiyi,Xi Yebing,Chen Guangjie,Chou Kuangyan.Fas Ligand Expressed on HEpG2 Cells Induces the Cytotoxicity of T Lymphocytes[J].Current Immunology,2000,20(5):260-262. |
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| Authors: | Feng Di Li Weiyi Xi Yebing Chen Guangjie Chou Kuangyan |
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| Institution: | Feng Di
,Li Weiyi
,Xi Yebing
,Chen Guangjie
,Chou Kuangyan
;(Shanghai Institute of Immunology , Shanghai Second Medical University, Shanghai 200025) |
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| Abstract: | To investigate the possibility of tumor cells to escape from immune surveillance by the expression of FasL on tumor cells a study was undertaken by using RT-PCR,immunochemistry and the detection of apoptosis It was found that FasL was expressed on HEpG2 cells at mRNA and protein levels,but not on the 7721 cells of liver cancers When the HEpG2 cells were co-cultured with the Jurkat T cells,apoptosis of the latter cells was observed in 19 4% of cases, and this could blocked by anti-FasL monoclonal antibody It concludes that FasL-expressing HEpG2 cells could inversely induce apoptosis of T lymphocytes |
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| Keywords: | tumor cells FasL apoptosis immunochemistry |
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