麻杏石甘汤对腺病毒感染的HELF细胞因子蛋白表达影响的实验研究

目的探讨麻杏石甘汤含药血清对3型腺病毒感染的人胚肺成纤维细胞（HELF）之转化生长因子-β1（TGF-β1）、血小板衍生生长因子-BB（PDGF-BB）、肿瘤坏死因子-α（TNF-α）蛋白表达的影响。方法以3型腺病毒攻击体外培养的HELF,制备麻杏石甘汤含药血清,作用于该细胞,另设正常细胞组,病毒对照组,利巴韦林组,采用酶联免疫吸附试验（ELISA法）检测不同组细胞TGF-β1、PDGF-BB、TNF-α的蛋白表达,并进行比较。结果病毒对照组较正常细胞组之TGF-β1、PDGF-BB、TNF-α蛋白表达均明显增强（P〈0.01）,含药血清组可显著降低3型腺病毒攻击后HELF的TGF-β1、PDGF-BB、TNF-α的蛋白表达（P〈0.01）。结论麻杏石甘汤可下调3型腺病毒感染后TGF-β1、PDGF-BB、TNF-α的蛋白表达,这可能是其治疗病毒性肺炎的作用机制之一。

Experimental Research of the Impacts of Maxing Shigan Tang on the Protein Expression of HELF Cellular Factors in Adenoviral Infection

Objective To explore the impacts of the medicated serum of maxing shigan tang on the protein expressions of transformation growth factor-β1（TGF-β1）,platelet-derived growth factor-BB（PDGF-BB）and tumor necrosis factor-α（TNF-α）in human embryo lung fibroblasts（HELF）after the infection of adenovirus type 3.Methods The adenovirus type 3 was used to attack the in vitro cultured HELF.The medicated serum of maxing shigan tang was prepared and worked on the cells.A normal cell group,a virus control group and a Ribavirin group were set up separately.The enzyme-linked immunosorbent assay（ELISA）was adopted to detect the protein expressions of TGF-β1,PDGF-BB and TNF-α in the cells of each group and the comparison was made.Results The protein expressions of TGF-β1,PDGF-BB and TNF-α in the virus control group were stronger apparently than those in the normal cell group（P〈0.01）.The protein expressions of TGF-β1,PDGF-BB and TNF-α of HELF after the attack of adenovirus type 3 were reduced significantly in the medicated serum group（P〈0.01）.Conclusion Maxing shigan tang down-regulates the protein expressions of TGF-β1,PDGF-BB and TNF-α of HELF after the infection of adenovirus type 3,which is probably one of the mechanisms of its efficacy on viral pneumonia.