胚胎干细胞联合碱性成纤维细胞生长因子移植对大鼠急性心肌梗死的影响

目的 探讨胚胎干细胞-D3株(ES-D3)联合碱性成纤维细胞生长因子(bFGF)移植治疗大鼠急性心肌梗死,是否有利于心脏结构的恢复和心功能的改善.方法 Wistar大鼠40只随机均分成5组,分别为正常对照组(组1)、梗死未治疗组(组2)、培养基注射组(组3)、ES-D3移植组(组4)、ES-D3 bFGF移植组(组5).大鼠急性心肌梗死造模后1周移植体外分化并经标记的ES-D3,4周后进行心功能及组织学检测.结果 ES-D3体外能分化为心肌样细胞.梗死后4周检测表明,移植细胞在大鼠体内稳定存活.心功能及组织学检测表明,组2与组3大鼠无显著差异(P>0.05).与组2比较,组4和组5大鼠心肌梗死面积均显著减小,左心室重量减轻(P<0.01);毛细血管密度显著增高(P<0.01);左心室功能显著改善.结论 急性心肌梗死后移植ES-D3可以促进大鼠心血管新生、阻止心室重构、减少瘢痕面积、显著改善心功能,联合应用bFGF可进一步获益.

THE EFFECT OF INTRAMYOCARDIAL TRANSPLANTATION OF EMBRYONIC STEM CELLS COMBINED WITH BASIC FIBROBLAST GROWTH FACTOR INTO MYOCARDIAL INFARCTION MODELS ON THE CARDIAC FUNCTION IN POSTINFARCTED RATS

Objective To investigate the feasibility of recovering the cardiac structure and improving the cardiac function after the transplantation of embryonic stem cells-D3(ES-D3) combined with basic fibroblast growth factor(b-FGF) into the acute myocardial infarction(AMI). Methods Forty Wistar rats were used and randomly divided into 5 groups equally,including the normal control group(group 1),the myocardial infarction without treatment group(group 2),the medium injected group(group 3),the ES-D3 transplanted group(group 4),the combination of ES-D3 and bFGF transplanted group(group 5).One week after the acute myocardial infarction(AMI),rats were injected with the in vitro differentiated ES-D3 labeled by BrdU.Then the cardiac function and histology were measured in week 4 after the transplantation. Results ES-D3 could differentiate into cardiomyocytes in vitro,and could survive steadily for 4 weeks in vivo after the transplantation into AMI's model.There were no significant difference between group 2 and group 3 in cardiac function(P>0.05).Compared with group 2,the infarction size and left ventricular weight(LVW) were reduced significantly(P<0.01)in group 4 and group 5,and capillary density was increased significantly(P<0.01).The functions of the left ventricle were remarkably improved.Conclusion The transplantation of ES-D3 can promote angiogenesis,improve the cardiac function,prevent the remodeling process of the left ventricle and reduce the infarct size,and further benefits can be obtained by co-administration of bFGF.