壬基酚对子代大鼠皮质部神经生长相关蛋白的影晌

目的母鼠孕期和哺乳期暴露NP，观察其对子代神经生长相关蛋白（GAP-43）的影响。方法将交配成功的31只孕鼠根据妊娠H期分层后随机分配到4组，即对照组，NP低、中、高剂量组（NP0、25、50、100mg／kg／day），单笼饲养，空腹灌胃，NP暴露时间为受孕第6天到出生后21d哺乳期结束（GD6-PND21），免疫组织化学法检测21d、60d皮质部GAP-43的变化，并分析上述变化与NP暴露之间的关系。结果高剂剂量组21d和60d龄的仔鼠皮质部GAP-43免疫反应阳性细胞平均数目、积分光密度低于同期对照组仔鼠（P〈0．05）；GAP-43蛋白表达与NP暴露剂量有负相关关系，差异有统计学意义（r=-0．711、-0．568，P〈0．05）。结论NP孕期暴露影响子代大鼠脑组织GAP-43的表达，且剂量越高，GAP-43表达越低。

Adverse effects of Nonylphenol on GAP- 43 expression in the brain cortex of rat pups

Objective To observe the adverse effects of Nonylphenol （NP） on neuronal growth - associated pro- tein （ GAP - 43 ） expression in the brain cortex of pups exposed to NP during gestational and lactational periods. Methods 31 pregnant dams were randomly assigned to 4 groups. The treatment groups received gavage with NP at dose levels of 25 mg/kg/day （low dose）, 50 mg/kg/day （middle dose）, 100 mg/kg/day （high dose） and groundnut oil alone （vehicle control）, respectively. The pregnant dams were housed individually and fed intra- gastrically. NP exposure time was limited from gestational day 6 to postnatal day 21. Alterations in GAP -43 in the brain cortex of rat pups at PND 21 and PND 60 was detected using immunohistochemistry, and the associa- tion between NP exposure and these alterations in GAP - 43 was analyzed. Results GAP - 43 - positive cells and integrated optical density （IOD） of pup cerebral cortex at PND 21 and 60 in high - dose NP treatment group were lower than the control offspring （P 〈 O. 05 ）. A negative and significant relationship between NP exposure dose and GAP-43 protein expression was revealed （ r = -0. 711, -0. 568 ,P 〈0.05 ）. Conclusion Exposure to NP during gestation might have an adverse effect on GAP -43 expression in the brain of pup rats, an inverse dose -response relationship was found between NP exposure and GAP- 43 expression.