内源性大麻素系统与神经胶质瘤细胞增殖与凋亡的关系分析

目的:探讨细胞实验分析内源性大麻素系统与神经胶质瘤细胞增殖与调亡的关系。方法:选择鼠C6细胞和鼠U251细胞及进行培养,采用MTT法测定大麻素四氢大麻酚(THC)对C6细胞增殖的影响,采用DNA梯度电泳法检测C6细胞凋亡,采用Western Blotting法检测CB1、CB2、重组人半胱天冬酶-3(caspase-3)和过氧化物酶体增殖物激活受体(PPAR)蛋白的表达。结果:大麻素受体CB1和CB2在C6细胞和U251细胞都有表达,对比差异无统计学意义(P>0.05)。不同浓度THC(0、1、10μmol/L)的细胞生长抑制率分别为0%、6.3%、29.3%,细胞凋亡率为5.2%、7.3%和12.7%,三组间的抑制与凋亡率对比差异有统计学意义(P<0.05)。THC不同浓度组(1、10μmol/L)C6细胞中Cleaved capase-3、PPAR蛋白表达均明显升高,与空白对照组(0μmol/L)比较差异均具有统计学意义(P<0.05)。结论:内源性大麻素系统在体外能抑制鼠C6细胞的增殖和促进其凋亡,其作用的发挥与caspase-3和PPAR表达有关。

The relationship between endocannabinoid system and glioma cell proliferation and apoptosis

Objective：Used the cell experiments to discuss the relationship between endocannabinoid sys-tem and glioma cell proliferation and apoptosis .Methods：Selected mouse C6 cells and U251 cells were cultured , used the MTT assay to detect C6 cell proliferation by the cannabinoids THC ,used the DNA gradient gel electropho-resis assay to detect C6 cell apoptosis ,used Western Blotting assay to detect the expression of CB1 ,CB2 ,caspase-3 and the PPAR protein .Results ：The CB1 and CB2 were expressed in the C6 cells and U251 cells that compared had no significant difference （P〈0 .05） .The different concentrations of THC （0 ,1 ,10 μmol /L） of cell growth in-hibition rates were 0 % ,6 .3% ,29 .3% ,and the apoptosis rate were 5 .2% ,7 .3 % and 12 .7% ,among the three groups of inhibition and apoptosis rates contrasted had significantly （P〈 0 .05） .The C6 cells Cleaved capase-3 , PPAR protein expression in the THC different concentrations （1 ,10 μmol /L）were significantly increased ,com-pared with the control group （0μmol /L） had statistically significant differences （P 〈0 .05） .Conclusion ：Endoge-nous cannabinoid system in vitro can inhibit cell proliferation and promote apoptosis in mouse C 6 cells ,its role is re-lated to the caspase-3 and PPAR expression .