烟曲霉提取物对人支气管上皮细胞嗜酸性粒细胞趋化因子浓度的影响

目的 探讨烟曲霉对呼吸道上皮细胞人嗜酸性粒细胞趋化因子（Eotaxin）浓度变化的影响及其可能机制,为研究烟曲霉在变应性支气管肺曲霉病发病机制中的作用奠定基础.方法 分别应用不同浓度（0、8、16、20 mg/L）的烟曲霉提取物（AFE）作用体外培养人支气管上皮细胞（16HBE） 不同的时间（12、24、48、72 h）.应用ELISA法检测细胞上清液Eotaxin浓度.结果 正常未给予AFE刺激培养条件下的对照组细胞表达一定量Eotaxin,而给予不同浓度的AFE作用24 h后,细胞合成和分泌Eotaxin的量明显高于对照组,差异有统计学意义（P〈0.01）,并呈明显的时间依赖性.热处理的AFE仍然能刺激16HBE细胞分泌Eotaxin并呈时间依赖性.随着AFE浓度的升高在相同作用时间下AFE刺激16HBE细胞分泌Eotaxin 的浓度并未呈现上升趋势.结论 AFE并非依赖其蛋白酶活性促进呼吸道上皮细胞合成和分泌Eotaxin,一定浓度的AFE刺激16HBE 细胞分泌Eotaxin呈明显的时间依赖性.

Effects of Aspergillus fumigatus extracts on the content of Eotaxin in human bronchial epithelial cells

Objective To investigate the effects of Aspergillus furnigatus extracts（AFE） on the content of Eotaxin in human bronchial epithelial cells and its possible mechanism,and to lay the foundation for the study on the effect of Aspergillus furnigatus in the pathogenesis of allergic bronchial pulmonary aspergillosis. Methods Human bronchial epithelial cells（16HBE） were cultured in vitro, which were exposed to different concentrations of AFE（0,8,16,20 mg/L） for different times （ 12,24,48,72 h）. The pro- duction and release of Eotaxin in different groups were tested by ELISA. Results In normal control group, certain amount of Eotaxin was detected. In the experimental groups with AFE exposure after 24 h, cells produced more Eotaxin compared to normal control group（P〈0.01）, which were positively related to the exposure time. Heat-treated AFE, which lost protease activities, also exerted effect on Eotaxin production. With the increase of AFE concentration, the stimulation of 16HBE cells secreting Eotaxin con- tent did not show a rising trend. Conclusion AFE is not dependent on its protease activity causes human bronchial epithelial cells to produce and release more Eotaxin. A certain concentration of AFE stimulation of 16HBE cells secreting Eotaxin is positively related to the exposure time.