Serum FDP assay with latex photometric immunoassay and its analysis by immunoblotting |
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| Authors: | N Okumura H Kagiya H Saito T Katsuyama M Kanai |
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| Affiliation: | Central Clinical Laboratories, Shinshu University Hospital, Matsumoto. |
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| Abstract: | Sixty patients' sera with FDP-E value ranged from 200 to 2,500 ng/ml by LPIA system were assayed for FDP-Total (FDP-T) by the same system and FDP-DD by ELISA. Correlations of values between FDP-E, FDP-T and FDP-DD were excellent and ratio of FDP-T/FDP-E (T/E) and FDP-DD/FDP-E (DD/E) from same 60 sera were 32.4 + 7.4 and 1.55 + 0.50, respectively. Ratio of T/E from sera of FDP-E value demonstrating more than 500 ng/ml were classified into three groups, namely, 25.0-35.0, less than 18.0, and more than 50.0. Ten sera in each group were analyzed for FDP fragments of D, DD, Y, DY or X (DY/X) and high molecular weight (HMW) with SDS-PAGE and immunoblot method. It was found that relative concentration and proportion of D and DD.E fragments showed its characteristic pattern in each group. |
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