Nucleotide sequence of the gene encoding the Newcastle disease virus hemagglutinin-neuraminidase protein and comparisons of paramyxovirus hemagglutinin-neuraminidase protein sequences |
| |
| Authors: | L W McGinnes A Wilde T G Morrison |
| |
| Affiliation: | 1. Recombinant DNA Lab, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243 122, India;2. Institute of Marine & Environmental Technology, University of Maryland Baltimore County, Baltimore, USA;1. Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, Neston, Cheshire CH64 7TE, UK;2. Department of Animal Health, Animal Wealth Directorate, Ministry of Agriculture and Fisheries, Oman;3. National Institute of Health Research Health Protection Research Unit in Emerging and Zoonotic Infections, Liverpool, UK;1. Laboratório de Vírus Respiratórios e do Sarampo, National Influenza Center (NIC)/ World Health Organization (WHO), Instituto Oswaldo Cruz/Fiocruz, Rio de Janeiro, Rio de Janeiro, Brazil;2. Laboratório Central de Saúde Pública do Estado do Paraná, Curitiba, Paraná, Brazil;3. Laboratório Central de Saúde Pública do Estado de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil;4. Laboratório Central de Saúde Pública do Estado do Espírito Santo, Vitória, Espírito Santo, Brazil;5. Laboratório Central de Saúde Pública do Estado de Alagoas, Maceió, Alagoas, Brazil;6. Centro de Transplante de Medula Óssea, Instituto Nacional de Câncer, Rio de Janeiro, Rio de Janeiro, Brazil;7. Influenza Division, National Center for Immunization and Respiratory Diseases, CDC, Atlanta, USA;8. WHO Collaborating Centre for Reference and Research on Influenza, VIDRL at the Peter Doherty Institute, Melbourne, Victoria, Australia |
| |
| Abstract: | The nucleotide sequence of cloned cDNA copies of the mRNA encoding the Newcastle disease virus (NDV), strain A-V, hemagglutinin-neuraminidase (HN) protein was determined. A single open reading frame in the sequence encodes a protein of 570 amino acids with a calculated molecular weight of 62,280. The predicted protein sequence contains only one obvious potential membrane spanning region, located 27 amino acids from the amino terminus of the sequence. The predicted sequence contains 6 glycosylation sites and 14 cysteine residues. Comparison of the NDV HN protein sequence with three other paramyxovirus HN protein sequences reveals two regions that have homologies in all four sequences. The conserved cysteine residues are clustered in these two regions. One conserved region is located near the middle of the predicted sequence while the second region is in the carboxy terminal third of the molecule. The presence of conserved regions suggests the importance of these areas of the molecule in the structure or function of the protein. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
