慢性再生障碍性贫血中医辨证分型与MHC-DRB1*等位基因的相关性研究

目的:探讨慢性再生障碍性贫血中医辨证与MHC-DRB1等位基因多态性之间的关系.方法:采用聚合酶链式反应/序列特异性引物的技术方法,对慢性再生障碍性贫血(CAA)患者和对照组的MHC-DRB1各等位基因进行检测和分析.结果:肾阳虚型组患者MHC-DRB10301基因频率明显高于健康对照组,两组差异有非常显著性(P＜0.01).MHC-DRB1各等位基因未见与CAA肾阴虚型相关.结论:MHC-DRB10301基因可能是CAA肾阳虚型的易感基因,或者也可能是CAA的易感基因或其一部分,表明该型患者的免疫调控缺陷遗传基础的存在.MHC与CAA的先天体质疾病类型之间有一定的内在联系,能在一定程度上反映中医体质的遗传特征和CAA中医辨证的客观性.

Study on Correlativity of TCM Syndrome Types of Chronic Aplastic Anemia with MHC-DRB1 * Allelic Genes

Objective: To approach to relation of TCM syndrome differentiation of chronic aplastic anemia (CAA) with polymorphism of MHC-DRB1 * allelic genes. Methods: MHC-DRB1 * allelic genes in patients of CAA and healthy control group were detected and analyzed by PCR/sequence-specific primer technique. Results: MHC-DRB1 * 0301 gene frequency in the patient of kidney-Yang deficiency was significantly higher than that in the healthy control group (P<0. 01). MHC-DRB1 * allelic genes were not related with kidney-Yin deficiency in the patient of CAA. Conclusion: MHC-DRB1 * 0301 genes are possibly susceptible genes of kidney-Yang deficiency in the patient of CAA. or susceptible genes of CAA or a part of them, indicating that patients of this type have genetic basis of immunoregulative defect; and there is a certain inner link between MHC and disease types of congenital constitution of CAA. which can reflect genetic characteristics of TCM costitution and objectiveness of CAA TCM syndrome differentiation to a certain extent.