5-羟色胺转运体在模拟高原肺动脉高压大鼠肺组织中的基因表达及氟西汀的干预作用

目的 观察5-羟色胺转运体(5-HTT)在高原肺动脉高压大鼠肺组织中的基因表达规律,探讨氟西汀对其肺组织中5-HTT表达的干预作用.方法 84只雄性Wistar大鼠随机分为对照10 d,20 d,30 d组(C10,C20,C30组,各8只),高原低压低氧10 d,20 d和30 d组(H10,H20和H30组,各10...

Expression of 5-HIT mRNA in the lung tissue of the rats with simulated high altitude pulmonary hypertension and the intervention effects of fluoxetine

Objective To observe the expression of 5-hydroxytryptamine transporter (5-HTT) in the lung tissue of the rats with high altitude pulmonary hypertension (HAPH) and explore the intervention effects of fluoxetine. Methods 84 male Wistar rats were randomly divided into control groups (C10, C20, C30, n = 8), high altitude groups ( H10, H20, H30, n = 10), and fluoxetine treated groups (F10, F20 ,F30, n =10), the group C were fed in normal conditions,the group H and group F were fed in a hypobaria and hypoxia chamber simulated to an altitude of 5 000 meters. In addition, the group F rats were gastric gavaged with fluoxetine(3mg·kg-1·d-1),the group C and group H rats were gastric gavaged with distilled water by weight. The expression level of 5-HTT mRNA were examined by RTPCR in lung tissue. Results Compared with C10, C20, C30, the expression of 5-HTT mRNA was upregulated in the group H10[(0. 33±0. 05) vs (0. 12±0.02).(0. 13±0.01).(0. 12±0.04), P <0.01],and its expression increased with the extension of time in the hypobaria and hypoxia chamber H20(0. 63±0. 02), H30 (0.91±0. 03). Compared with H10, H20, the expression level of 5-HTT mRNA were significantly lower in F10, F20, ( P <0.01), but there are no difference between F30 and H30 [(0.93±0.01) vs (0.91 ±0.03), (P > 0.05)]. Conclusions The expression level of 5-HTT mRNA was upregulated in the lung tissue of the rats with HAPH. Fluoxetine can inhibit the expression of 5-HTT mRNA in the lung tissue for short-term, but its effect is not obvious for long-term.