宫颈癌患者血清和组织中胰岛素样生长因子-Ⅱ的表达及临床意义

的检测宫颈癌患者血清胰岛素样生长因子-Ⅱ（IGF-Ⅱ）水平及其在肿瘤组织中的表达，探讨IGF-Ⅱ在宫颈癌发生、发展中的作用。方法采用放射免疫法检测血清IGF-Ⅱ水平，应用免疫组织化学方法检测IGF-Ⅱ在正常宫颈组织和宫颈癌组织中的表达情况，同时对组织中IGF-Ⅱ表达与宫颈癌各临床病理参数之间的关系进行分析。结果（1）宫颈癌组和对照组血清IGF-Ⅱ浓度分别为（1．98±0．53）和（0．51±0．16）μg／L，二者相比差异有统计学意义（P〈0．01）；宫颈癌患者治疗后血清IGF-Ⅱ浓度比治疗前明显降低，淋巴结转移组血清IGF-Ⅱ浓度为（2．26±0．32）μg／L，明显高于淋巴结未转移组（1．734-0．42）μg／L，差异有统计学意义（P〈0．01）。（2）宫颈癌、正常宫颈组织中IGF-Ⅱ表达阳性率分别为73．3％和10．0％，二者相比差异有统计学意义（P〈0．01）。IGF-Ⅱ的阳性表达与宫颈癌临床分期、分化程度呈显著相关，差异有统计学意义（P〈0．01），其在淋巴结转移组的阳性表达率明显高于无淋巴结转移组（x^2=8．69，P〈0．01）。结论IGF-Ⅱ的高表达与宫颈癌的发生、发展及侵袭转移有关，检测IGF-Ⅱ能为宫颈癌的临床诊断和预后观察提供有意义的参考。

Variation of insulin-like growth factor Ⅱ in serum and tissue of patients with cervical cancer and its clinical significance

Objective To investigate the expression of serum and tissue insulin-like growth factor Ⅱ （IGF-Ⅱ ） in patients with cervical cancer, and to evaluate its clincial significance in the ocuurence and development of cervical cancer. Methods The serum level of IGF- Ⅱ was examined by radioimmunoassay （RIA）, and the expression level of IGF- Ⅱ in the tumor and normal tissue was detected with immunohistoehemical technique. The relationship of tissue expression level of IGF-Ⅱ and the clinicopathological parameters was analyzed as well. Results The serum IGF-Ⅱ levels in cervical cancer group and the control group were （1.98±0.53）μg/L and （0.5±0.16）μg/L, respectively. There was significant difference between them （P〈0.01） ; the serum IGF- Ⅱ level was decreased significantly after therapy. The serum IGF- Ⅱ level in lymph node metastasis group was obviously higher than that in lymph node nonmetastasis group （2.26±0.32）μg/L vs （1.73±0.42） μg/L,P〈0.01]. The positive expression rate of IGF-Ⅱ in cervical cancer tissues was 73.3%, significantly higher than that in normal cervical tissues （10.0%） （P〈0.01）. The positive expression of IGF- Ⅱ was closely correlated with different clinical stage and differentiation degree of carcinoma （P〈0.01）. The positive expression rate of IGF-Ⅱ in lymph node metastasis group was significantly higher that in lymph node non-metastasis group （2 = 8.69, P〈0.01）. Conclusion The excessive expression of IGF-Ⅱ may be correlated with the genesis, invasion and metastasis of cervical cancer. The expression of IGF- Ⅱ may be useful marker of diagnosis and prognosis evaluation of cervical cancer.