| 人脐血间质干细胞在脑创伤大鼠脑内移植后的迁移 |
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| 引用本文： | 宋来君,张鹏,杨波,杜英,关方霞,孙剑瑞,万鼎铭,胡祥,胡平生. 人脐血间质干细胞在脑创伤大鼠脑内移植后的迁移[J]. 郑州大学学报(医学版), 2006, 41(2): 230-233 |
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| 作者姓名： | 宋来君 张鹏 杨波 杜英 关方霞 孙剑瑞 万鼎铭 胡祥 胡平生 |
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| 作者单位： | 郑州大学第一附属医院神经外科,郑州,450052;郑州大学基础医学院微生物与免疫学教研室,郑州,450052;郑州大学,郑州,450052;郑州大学第一附属医院血液科,郑州,450052;深圳市北科细胞工程研究所,深圳,518000;瑞典Biovitron公司,999027 |
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| 基金项目： | 河南省医学科技人才创新工程项目 |
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| 摘 要： | 目的:研究人脐血间质干细胞(UCB-MSCs)脑内移植后的分布和移行,为细胞移植治疗脑创伤奠定基础.方法:健康孕妇顺产脐血经分离、培养得到人UCB-MSCs,并用Hoechst33258标记后备用.自由落体撞击法制作的大鼠脑创伤模型,将已标记的人UCB-MSCs移植到大鼠的脑损伤区.一段时间后处死大鼠,取脑组织切片,直接在荧光显微镜下观察移植细胞的存活和迁移.结果:细胞移植到大鼠脑内能够长时间存活,移植细胞与宿主细胞有很好的相容性,移植后3周细胞发出蓝白色光,沿移植道呈条带状分布,细胞在条带中央较为密集,在条带周围细胞分布逐渐稀疏.移植后6周,损伤组织中可见荧光标记的细胞,细胞分布较3周时稀疏.结论:人UCBMSCs脑内移植后,能够成活并向脑损伤区迁移,与宿主脑组织整合在一起.
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| 关 键 词： | 脐血 间质干细胞 培养 移植 迁移 大鼠 |
| 收稿时间： | 2005-09-28 |
| 修稿时间： | 2005-09-28 |
Migration after human umbilical cord blood mesenchymal stem cells transplantation in brain injury rats |
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| SONG Laijun,ZHANG Peng,YANG Bo,DU Ying,GUAN Fangxia,SUN Jianrui,WAN Dingming,HU Xiang,HU Pingsheng. Migration after human umbilical cord blood mesenchymal stem cells transplantation in brain injury rats[J]. Journal of Zhengzhou University: Med Sci, 2006, 41(2): 230-233 |
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| Authors: | SONG Laijun ZHANG Peng YANG Bo DU Ying GUAN Fangxia SUN Jianrui WAN Dingming HU Xiang HU Pingsheng |
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| Affiliation: | 1 . Department of Neurosurgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 ;2 . Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052; 3. Visiting Professor, Department of Biomedicine and Bioengineering, Zhengzhou University, Zhengzhou 450052; 4. Department of Hematology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052; 5. Shenzhen Beike Cell Engineering Institute, Shenzhen 518000; 6. Biovitron Company, Sweden 999027 |
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| Abstract: | Aim: To explore the migration and location of human umbilical cord blood mesenchymal stem cells (UCB-MSCs) after they were transplanted into adult brain tissue and to investigate the possibility of a new cell source for central nervous system transplantation.Methods:UCB-MSCs were derived from separated umbilical cord blood, then, cultured and labeled by Hoechst33258 and were stereotactically transplanted into adult rat striatum after experimental traumatic brain injury. After a period of time, the rats were sacrified and their brain tissue was sectioned to identify the transplanted cells under fluorescent microscope.Results: These cells lived well and showed good compatibility when they were grafted into adult brains. Three weeks after transplantation, the marked UCB-MSCs were giving out blue-white lights and the morphology was intact and cells were spreading along the two sides of needle canal. The nearer to canal,the denser to spread. At 6 weeks after transplantation, the distribution of cells near the canal was sparser than that of 3 weeks.Conclusion: UCB-MSCs could live and incorporate into host brains when they are transplanted into adult brain tissue. |
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| Keywords: | umbilical cord blood mesenchymal stem cell culture transplantation migration rat |
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