内源性睾酮抑制对大鼠睾丸内α-catenin表达的影响

目的:检测十一酸睾酮注射致内源性睾酮抑制的大鼠睾丸内α-caten in的表达情况。方法:成年雄性SD大鼠10只,随机分为对照组(肌注生理盐水)和睾酮组[肌注十一酸睾酮19 mg/(kg.15 d)],共130 d。制作睾丸石蜡切片,采用抗α-caten in多克隆抗体进行免疫组化染色。结果:在对照组中,α-caten in主要表达于精子细胞顶体、管周肌样细胞和Leyd ig细胞胞质。睾酮组的Leyd ig细胞明显萎缩,-αcaten in的表达明显减弱或消失,而精子细胞顶体和管周肌样细胞胞质的-αcaten in表达无明显改变。结论:内源性睾酮抑制所致生精细胞排列疏松或脱落与粘附分子-αcaten in的表达无关。-αcaten in有可能成为识别Leyd ig细胞的标记物。

Effect of Intra-testicular Testosterone Withdrawal on the Expression of α-catenin in the Adult Rat Testis

Objective: To study the expression of α-catenin in the rat testis after intra-testicular testosterone withdrawal induced by injection of testosterone undecanoate(TU).Methods: Ten adult male SD rats received vehicle(n=5) or TU(19 mg/kg every 15 days,n=5) for 130 days.Paraffin-embedded testicular sections were used for immunohistochemistry against a polyclonal anti-α-catenin antibody.Results: In the control,α-catenin was expressed in the acrosome of spermatids and the cytoplasm of Leydig cells and peritubular myoid cells.In the TU-treated rat testis,Leydig cells were atrophied and the expression of α-catenin was markedly decreased or absent,but there was no evident change in the immunostaining of spermatids or myoid cells.Conclusion: Intra-testicular testosterone withdrawal-induced looser arrangement or sloughing of spermatogenic cells is not related to the adhesion molecule α-catenin.α-catenin may be used as a cell identification marker for Leydig cells.