酶联免疫吸附试验和直接免疫荧光检测沙眼衣原体的方法学比较

目的：评价酶联免疫吸附试验（ELISA）和直接免疫荧光（DFA）法检测沙眼衣原体（CT）的灵敏度和特异性。方法：分别采用CT抗原DFA和ELISA法检测泌尿生殖道CT，两法结果不一致时采用荧光定量PCR确证。以DFA和ELISA法均阳性或经PCR确证阳性的结果作为真阳性，分别计算DFA和ELISA法的灵敏度、特异性、阳性预测值和阴性预测值。结果：共检测222份女性宫颈拭子和69份男性尿道拭子，真阳性66例，真阴性225例，其中DFA和ELISA检测结果均阳性63例，另10例两法结果不符的样本经PCR检测阳性3例。ELISA法的灵敏度、特异性、阳性预测值和阴性预测值分别为97．0％、98．7％、95．5％和99．1％，而DFA分别为98．5％，98．2％，94．2％，99．5％。两种方法检测结果符合率为96．6％。结论：ELISA和DFA法检测CT有较高的灵敏度和特异性，均可为临床治疗和性病监测工作提供可靠依据。

Comparison of enzyme-linked immunosorbent assay and direct fluorescent antibody for the detection of Chlamydia trachomatis

Objective:To investigate the sensitivity and specificity of the enzyme-linked immunosorbent assay(ELISA) and direct fluorescent antibody(DFA) for the detection of Chlamydia trachomatis. Methods:291 genital specimens were collected from 222 females and 69 males.DFA and ELISA were used for the detection of Chlamydia trachomatis and PCR for the samples with inconsistent results by DFA and ELISA.Sensitivity,specificity,positive predictive value and negative predictive value of ELISA and DFA were analyzed.Result...