| HPLC测定葛根中葛根素和大豆苷的含量 |
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| 引用本文： | 凌樱珊,胡向全,李文楷,吴方评. HPLC测定葛根中葛根素和大豆苷的含量[J]. 中国民族民间医药杂志, 2012, 0(9): 31-33 |
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| 作者姓名： | 凌樱珊 胡向全 李文楷 吴方评 |
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| 作者单位： | 湖南省怀化医学高等专科学校,湖南怀化418000 |
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| 摘 要： | 目的：优选色谱条件，用高效液相色谱法测定葛根中葛根素和大豆苷含量。方法：以乙腈（A）和二次蒸馏水（内含0．05％磷酸、0．05％三乙胺）（B）为流动相，流速0．5mLmin-1，检测波长为210nm，进行梯度洗脱。结果：葛根素、大豆苷与其它杂质的分离度较好，葛根素和大豆苷的标准回收率分别为95．7％和95．6％，相对标准偏差分别为2．1％和1．7％。结论：方法可用于葛根中葛根素和大豆苷的测定。
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| 关 键 词： | 高效液相色谱 葛根 葛根素 大豆苷 |
Study on Determination of Puerarin and daidzin inPuerariae Lobatae Radix by High Performance Liquid Chromatography Method |
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| Affiliation: | Ling Ying - shah, Hu Xiang - quan, Li Wen - kai, Wu Fang - ping Huai hua medical college huihua hunan 418000, China |
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| Abstract: | Objective： High performance liquid chromatography method （HPLC） for the determination of puerarin in puerariae lobatae radix by optimizing chromatography conditions was studied. Method： The mobile phase consisted of acetonitrile （A） - water （ containing 0.05% H3PO4 and 0. 05% three ethylamine） . The flow rate was 0.5mL min - 1. Puerarin and daidzin was monitored at 210nm by gradient elution. Result： Separating degree of puerarin , daidzin and impurities was better. The recoveries of puerarin and daidzin were 95. 7% and 95.6%. The relative standard deviations were 2. 1% and 1.7%. Conclusion： The method was applied to the determination of puerarin and daidzin in puerariae lobatae radix with good result. |
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| Keywords: | HPLC puerarin daidzin puerariae lobatae radix |
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