个体化钛支架复合珊瑚羟基磷灰石和重组人骨形成蛋白2修复兔下颌骨缺损

目的通过观察个体化钛支架复合珊瑚羟基磷灰石(CHA)和重组人骨形成蛋白2(rhBMP-2)修复兔下颌骨缺损的实验效果,探讨下颌骨个体化修复再生的途径。方法以9只新西兰大白兔为实验对象,采用磨骨术建立兔下颌骨单侧缺损模型,利用计算机辅助设计/制作、快速成型技术等设计制作兔下颌骨个体化钛修复体,再将其与CHA和rhBMP-2复合应用于兔下颌骨标准化缺损修复,通过大体标本观察、骨密度检测、生物力学测试和组织学染色对下颌骨缺损的个体化再生修复效果进行研究。结果兔下颌骨解剖形态恢复理想,生物力学测试、骨密度检测及组织学观察均显示随时间点延长成骨效果呈明显上升趋势(P<0.05),钛支架内具有大量新骨形成,新生骨组织在24周时已明显成熟。结论采用快速成型技术制作的个体化钛支架复合CHA和rhBMP-2,可望通过骨再生途径实现下颌骨缺损的个体化修复重建。

The experimental study on the effect of repairing mandibular defects with CHA/rhBMP-2 by individualized titanium bracket

Objective To study the effect of individualized titanium bracket combined with coralline hydroxyapatite(CHA) and recombinant human bone morphogenetic protein-2(rhBMP-2) on bone defect repairing.Methods Radial critical size bone defect models were made on 9 rabbits,Individualized titanium bracket were obtained from CAD/CAM and RP.X-ray,histological examination,biomechanics analysis and immunohistochemical staining were made 4,12,and 24 weeks post operation.Results The integrity of the mandible was ideally recovered.New bone formation was seen in both the X-ray observation and the histological examination(P < 0.05).The best osseo-integrity can be observed at 24th week.Conclusion It is possible to obtain individualized functional reconstruction of mandibular segmental defect using individualized three-dimensional titanium bracket combined with CHA and rhBMP-2.