天津市媒介动物自然感染肾综合征出血热病毒的调查

目的搞清天津市肾综合征出血热在媒介生物种群中的带毒情况,为防制出血热提供科学依据.方法2002年2月～2004年8月对天津市部分地区进行了媒介动物自然感染肾综合征出血热病毒的分离研究工作.采用间接免疫荧光技术进行鼠肺检测,阳性鼠肺用Vero-E6细胞进行病毒分离.结果共捕集媒介动物鼠类450只,鼠种抗体总阳性率为3.55%.其中秋季阳性率为5.75%,春季阳性率为3.83%,夏季为0.98%.对出血热病毒抗体阳性的16只鼠类采用VeroE6细胞进行了病原分离研究,分离出病毒8株,分离成功率为50%,其中从褐家鼠中分离出病毒4株,社鼠2株,小家鼠2株;从鼠体捕集到2批螨类72只,采用Vero-E6细胞培养分离出病毒1株.结论天津市目前主要是以家鼠型为主的出血热疫区,可能存在多型别出血热病毒,出血热防制工作重点应放在春秋两季,同时要进一步探讨螨类与出血热的关系.

HFRSV Natural Infection among Intermedium Animals of Tianjin

Objective To learn the HFRSV natural infection among intermedium animals of Tianjin and provide scientific basis for the prevention work. [Methods] Indirect immune fluorescent technology was applied to detect the rats' lungs; the positive HFRSV was separated with Vero-E6 cells. [Results] The total positive rate of the 450 rats was 3.55%, among which 5.75% were found in autumn, 3.83% in spring and 0.98% in summer. 8 strains of HFRSV were successful separated (50%). 72 acarids were caught from the rats and 1 strain of virus was separated with Vero-E6. [Conclusion]HFRSV in Tianjin is mainly related with rats. Prevention work should be strengthened in spring and autumn.