| ＨＭＧＢ１表达载体转染结肠癌细胞对ＶＥＧＦ-Ｄ表达的影响 |
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| 引用本文： | 李焱,潘凤,何建民,李建军,梁后杰.ＨＭＧＢ１表达载体转染结肠癌细胞对ＶＥＧＦ-Ｄ表达的影响[J].临床肿瘤学杂志,2009,14(4):289-292. |
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| 作者姓名： | 李焱 潘凤 何建民 李建军 梁后杰 |
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| 作者单位： | 1 第三军医大学西南医院肿瘤科
2 成都军区总医院肿瘤诊治中心 |
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| 摘 要： | 目的：构建ＨＭＧＢ１表达载体，转染结肠癌细胞，研究其对结肠癌细胞中血管内皮生长因子Ｄ（ＶＥＧＦ-Ｄ）表达的影响。方法：将分离得到的淋巴细胞总ＲＮＡ反转录合成ｃＤＮＡ，以此为模板进行ＰＣＲ扩增得到ＨＭＧＢ１基因；随后酶切转入载体ｐＭＤ１８Ｔ，通过亚克隆转入载体ｐＬｘｓｎ，得到重组质粒。重组体质粒经酶切鉴定后，并对插入的ＨＭＧＢ１基因片段进行测序，将已鉴定的阳性重组质粒用脂质体介导转染ＨＣＴ１１６细胞。通过ＲＴ-ＰＣＲ和Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ检测ＨＭＧＢ１和ＶＥＧＦ-Ｄ的表达情况。结果：成功构建含ＨＭＧＢ１的表达载体。ＲＴ-ＰＣＲ和Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ检测发现转染表达ＨＭＧＢ１载体的ＨＣＴ１１６细胞中ＨＭＧＢ１和ＶＥＧＦ-Ｄ的表达均增高。结论：ＨＭＧＢ１可以通过促进结肠癌细胞中ＶＥＧＦ-Ｄ的表达，诱导淋巴管生成，从而促进其淋巴结转移。
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| 关 键 词： | 高迁移率族蛋白1 血管内皮生长因子D 淋巴管生成 淋巴道转移 结肠癌 |
| 收稿时间： | 2008-12-10 |
| 修稿时间： | 2009-01-08 |
The change of VEGF D expression after the transfection of HMGB1 expression vector into colon cancer cell |
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| LI Yan,PAN Feng,HE Jian-min,LI Jian-jun,LIANG Hou-jie.The change of VEGF D expression after the transfection of HMGB1 expression vector into colon cancer cell[J].Chinese Clinical Oncology,2009,14(4):289-292. |
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| Authors: | LI Yan PAN Feng HE Jian-min LI Jian-jun LIANG Hou-jie |
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| Institution: | LI Yan,PAN Feng,HE Jian-min,LI Jian-jun,LIANG Hou-jie.Department of Oncology,Southwest Hospital,the Third Military Medical University,Chongqing 400038,China |
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| Abstract: | Objective:To construct the expression vector pLxsn-HMGB1 and transfect the recombinant vector into colon cancer cell in order to investigate the change of expression of VEGF-D.Methods:HMGB1 was obtained by RT-PCR from lymphocytes and was cloned into vector pMD18T firstly,then subcloned into vector pLxsn.The recombinant vector was verified by restriction enzyme digestion and sequencing.The positive recombinant plasmid was transferred into HCT116 cells using Lipofectamine 2000.The expressions of gene HMGB1 an... |
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| Keywords: | HMGB1 VEGF-D Lymphangiogenesis Lymph node metastasis Colon cancer |
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