大鼠胆道梗阻后肝细胞线粒体钙含量变化及粉防已碱的保 …

目的 观察肝细胞一体钙含量变化在胆道梗阻所致肝损伤的作用及粉防已碱的保护作用。方法 复制大鼠胆道梗阻模型，灌胃给予粉防已碱（Ｔｅｔ）３０ｍｇ．ｋｇ＾－１．ｄ＾－１，分离肝细胞线粒体，动脉观测肝细胞线粒体钙含量，血清Ｔ－Ｂｉｌ、ＡＬＴ、ＡＬＰ及ＣＧＴ含量变化。并观察肝脏显微结构改变。结果 胆道梗阻搏上细胞线粒体钙含随梗阻时间延长而逐渐升高，各梗阻组与对对照组比较，Ｐ〈０．０５；血清Ｔ－Ｂｉｌ、ＡＬＴ

Changes of calcium content of hepatocytic mitochondria and protective effects of tetrandrine after biliary obstruction in rats

Objective To investigate the role of the changes of hepatocytic mitochondrial calcium content and the protective effects of tetrandrine (Tet) on hepatic injury after biliary obstruction in rats. Methods After the model of complete biliary obstruction (CBO) was established in rats, Tet was administered once a day by gavage at a dose of 30 mg/kg. The contents of mitochondrial calcium and serum T-Bil, ALT, ALP and GGT were measured in each group. Hepatic morphological changes were observed under the light microscope. Results After CBO, the content of mitochondrial calcium was increased progressively (P<0.01) and those of serum T-Bil, ALT, ALP and GGT increased significantly (P<0.05). Light microscopy showed that hepatic injury was intensified progressively. The content of mitochondrial calcium was highly correlated with serum ALT and ALP. Conclusion After CBO, the calcium overload of mitochondria is intensified progressively. This may be one of the important mechanisms of hepatic injury. Tet can effectively protect the liver against the injury due to biliary obstruction.