肾上腺素对巨噬细胞吞噬和分泌功能的影响

目的 观察肾上腺素对巨噬细胞吞噬和分泌功能的影响.方法 收集大鼠腹腔巨噬细胞,采用不同浓度肾上腺素(E)(2～100μg/L)刺激1 h或10μg/L肾上腺素刺激不时间,检测细胞趋化、黏附、吞噬功能和培养上清TNF-α的浓度.结果 低浓度肾上腺素处理时,巨噬细胞趋化、黏附、吞噬功能均明显增强,细胞分泌TNF-α明显增多,其中以10μg/L肾上腺素处理时细胞功能增强最明显.但是随着肾上腺素浓度进一步增加,巨噬细胞功能未见进一步增强.时效实验结果显示,不同时间处理(0.5～5.0)h可不同程度地增强巨噬细胞趋化、黏附、吞噬和分泌TNF-α的功能,其中以处理1 h作用最明显.结论 适度肾上腺素刺激可明显增强巨噬细胞免疫功能.

The effects of epinephrine on phagocytosis and secretion functions of rat peritoneal macrophages

Objective To observe the modulatory effects of epinephrine (E) on the phagocytosis and secretion functions of maerophages.Methods After collection of peritoneal maerophages from rats,the cells were stimulated for 1 h by epinephrine at different concentrations ranging from 2 μg/L to 100 μg/L,or stimulated with 10 μg/L epinephrine for different durations.The immune functions of macrophages were evaluated,including chemotaxis,adherence,phagocytosis and the TNF-α levels in cell culture supernatants.Results When stimulated with epinephrine at low concentrations,the chemotaxis,aherence and phagocytic capacities of macrophages were enhanced obviously,and TNF-α secretion was improved significantly.The immune functions of maerophages were enhanced mostly by 10 μg/L epinephrine,However,such functions of macrophage were not enhanced further more when treated by higher concentrations of epinephrine.Besides,the time-effect experiments showed that within the treating time ranging from 0.5 h to 5.0 h by 10 μg/L epinephrine,all of the chemotaxis,adherence,phagocytic capacities of macrophages and the TNF-α levels in cell culture supernatants were improved to varying degrees,and the effects were the most after 1 h stimulation.Conclusion Acute stimulation of the epinephrine at a suitable concentration can enhance the immune functions of macrophages.