西格列汀对肥胖大鼠代谢指标及脂肪因子chemerin脂联素水平的影响

目的 研究西格列汀对肥胖大鼠代谢指标及脂肪因子chemerin、脂联素(ADPN)水平的影响及其可能机制,并探讨chemerin及ADPN与肥胖的关系。方法 将30只大鼠随机分为正常对照组(NC组)、肥胖对照组(HF组)和肥胖西格列汀干预组(SP组),ELISA法分别测定干预前后各组大鼠生化指标,Western blot 法检测大鼠脂肪、肝脏、肌肉及肾脏组织chemerin及ADPN蛋白表达水平。结果 与NC组相比,HF组大鼠血清chemerin、体质量(BW),空腹血糖(FBG)、胰岛素(FINS)、总胆固醇(TG)、甘油三酯(TC)、低密度脂蛋白胆固醇(LDL-C),胰岛素抵抗指数(HOMO-IR)增高,血清ADPN及高密度脂蛋白胆固醇(HDL-C)降低(P<0.05),HF组大鼠脂肪、肝脏、肌肉及肾脏组织的chemerin蛋白表达量较NC组对应组织的表达量增加(P<0.05),脂肪、肝脏及肌肉组织的ADPN表达量减少(P<0.05)。与HF组相比,SP组大鼠FBG、FINS、TC、TG、LDL-C、HOMO-IR、血清chemerin水平降低, HDL-C及血清ADPN水平升高(P<0.05),脂肪、肝脏、肾脏及肌肉组织的chemerin蛋白表达量减少,脂肪、肌肉组织的ADPN表达增加(P<0.05)。干预前chemerin、ADPN与BW、FBG、TG、HDL-C、LDL-C、FINS、HOMO-IR有相关性,干预后chemerin、ADPN与BW、LDL-C、FINS、HOMO-IR有相关性,ADPN与HDL-C呈正相关。结论 chemerin及ADPN参与糖脂代谢过程,西格列汀可改善血清学各代谢指标,并通过影响chemerin及ADPN来改善肥胖大鼠胰岛素敏感性。

Influence of sitagliptin on metabolic parameters and adipocytokines levels of obesity rats

Objective To investigate the effects of sitagliptin on metabolic parameters, chemerin and adiponectin levels of obesity rats, and to explore the relationship between the chemerin and adiponectin with obesity. Methods A total of 30 rats were randomly divided into normal diet group (NC), high-fat control group (HF) and sitagliptin intervention group (SP). The rats'' metabolic parameters were measured by ELISA.The chemerin and adiponectin protein expression levels in kidney adipose, liver and muscle tissue were detected by western blot. Results Compared with NC group, the serum chemerin, BW, FBG, FINS, TC, TG, LDL-C, HOMO-IR levels of high-fat model group were increased, HDL-C and adiponectin were decreased. Chemerin expression levels of high-fat control group in adipose, liver and kidney tissues increased compared with NC group, the expression of adiponectin in adipose, liver and muscle tissues was decreased. Compared with HF group, sitagliptin could down-regulate the levels of FBG, FINS, TC, TG, LDL, HOMO-IR, serum chemerin. The HDL-C and adiponectin were up-regulated. After sitagliptin treatment, chemerin expression levels in adipose, liver, kidney and muscle were reduced, and the expression of adiponectin in adipose and muscle tissue was increased. Correlation analysis showed that before intervention, chemerin and adiponectin were correlated with FBG, TG, HDL, LDL-C, FINS, HOMO-IR and BW. After intervention, chemerin and adiponectin had correlation with BW, LDL-C, FINS, HOMO-IR at the same time, adiponectin has positive correlation with HDL-C. Conclusion Chemerin and adiponectin may be a key participate element in the development of obesity. Sitagliptin can improve the metabolic indexes remarkably in serum, and improve insulin sensitivity in obesity rats by influencing the levels of chemerin and adiponectin.