| 胃癌患者外周血调节性T细胞检测及初步分析 |
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| 引用本文： | 乔治,李荣,陈凛,彭正,周国艮,梁文涛,徐飞,钟苑,徐迎新.胃癌患者外周血调节性T细胞检测及初步分析[J].中华实验外科杂志,2009,26(5). |
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| 作者姓名： | 乔治 李荣 陈凛 彭正 周国艮 梁文涛 徐飞 钟苑 徐迎新 |
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| 作者单位： | 解放军总医院普通外科,北京,100853 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的 检测胃癌患者外周血调节性T细胞(Treg)水平并对其进行初步分析.方法 四色流式细胞仪检测术前胃癌患者(n=25)及正常健康者(n=25)外周血CD4+ CD25+ FOXP3+Treg水平.结果 正常对照组CD4+ CD25+ FOXP3+ Treg/PBL为(1.229±0.656)%,胃癌组CD4+ CD25+ FOXP3+ Tres/PBL为(1.993±0.830)%.胃癌患者外周血Treg较正常对照组明显升高(P<0.01).结论 胃癌患者周血Treg水平升高,可因其免疫负调作用而导致免疫抑制.
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| 关 键 词： | 胃癌 调节性T细胞 流式细胞术 |
Detection and preliminary analysis of regulatory T cells in peripheral blood of gastric cancer patients |
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| Abstract: | Objective To investigate regulatory T cells in peripheral blood of gastric cancer pa-tients. Methods The CD4+ CD25+ FOXP3+ regulatory T cells in peripheral blood of gastric cancer pa-tients(n = 25)and normal individuals(n = 25) were assessed by four-color flow cytometric analysis. Re-suits The CD4+ CD25+ FOXP3+ Treg/PBL was(1. 229 + O. 656) % in normal control group, while that was(1. 993±0. 830) % in gastric cancer group. The number of CD4+ CD25+ FOXP3+ regulatory T cells in gastric cancer patients was significantly more than that in the normal control group (P < 0.01 ). Con-clusion There is an increased pool of CD4+ CD25+ FOXP3+ regulatory T cells in the peripheral blood of gastric cancer patients,which may be related to immunosuppression. |
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| Keywords: | Gastric carcinoma Regulatory T ceils Flow cytometry |
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