超声辐照对大鼠颈动脉损伤后早期c-jun,hsp70与BAX表达的影响

目的探讨超声辐照抗再狭窄作用的分子机制.方法建立大鼠颈动脉球囊损伤模型,予血管外超声辐照,采用原位杂交与免疫组化技术分别于术后30 min与4 h对血管壁-jun,hsp70,BAX(仅行免疫组化)的表达进行检测,对上述指标在术后以及超声辐照后的表达变化情况进行比较.结果原位杂交显示球囊损伤后30min-jun与hsp70 mRNA的表达显著增强,超声辐照降低了hsp70 mRNA在术后30 min的高表达(P＜0.01),对c-jun mRNA的表达无显著影响(P=0.773);术后4 h免疫组化结果与原位杂交结果基本吻合,同时发现超声辐照增强了术后4 h BAX蛋白在动脉壁中的表达(P＜0.05).结论该参数超声辐照减轻动脉损伤后再狭窄的作用机制可能与其在动脉损伤后早期减少hsp70的表达及增强BAX的表达有关,而与术后早期c-jun的高表达无明显关系.

Effects of ultrasound exposure on expression of c-jun,hsp70 and BAX in carotid artery of rats early after balloon lesions

Objective: To investigate the molecular mechanisms of the anti-restenosis effects of ultrasound exposure (USE). Methods: Balloon injured model of carotid artery of rats had been built with a 2F balloon catheter, then USE (705KHz, 1 w/cm2, 3 min) was applied. The expression of c-jun, hsp70 and BAX in the artery wall was detected by in situ hybridization (ISH) and Immunohistochemistry (IHC) 30 minutes and 4 hours after balloon lesions respectively. Results: The expression of c-jun and hsp70 mRNA in normal carotid artery increased significantly 30minutes after balloon lesions. There was no difference of c-jun mRNA expression between Balloon and Balloon+USE group (P =0.773), but the expression of hsp70 mRNA was significantly lower in the latter group (P ＜0.01). Results of the later IHC were similar with that of the ISH detection. The expression of BAX protein was significantly higher in Balloon+USE group than in Normal group (P ＜0.01) and Balloon group (P ＜0.05). USE alone couldn't change the expression of hsp70 and c-jun compared with Normal group. Conclusion: The anti-restenosis effects of the USE might be related to the changed expression of hsp70 and BAX in the damaged artery wall.