脾虚发热证大鼠模型的研究

目的:复制并评价脾虚发热大鼠模型。方法:大鼠随机分为正常对照组、单纯脾虚组、脾虚发热组。大鼠采用饮食失节+过度疲劳法复制脾虚模型,于造模第22天腹腔注射LPS(80μg/kg)诱导发热,测定各时间点的肛温、体重、外观行为变化积分值、尿D-木糖排泄率和外周血相关指标。结果:①与正常对照组比较,脾虚发热组大鼠体温除在30min有一过性降低外,其余各观察点均呈显著性升高(P0.01,P0.05);单纯脾虚组大鼠体温除在60min时有一过性升高外,其余各点无显著性差异;脾虚发热组与单纯脾虚组大鼠在第1、2、3周末的体重均明显降低;第2、3周末的外观行为变化总积分值呈显著性增加(P0.05,P0.01);第3周末的尿D-木糖排泄率、血GAS、MTLI、L-1β、IL-2、T3、T4、TSH均降低I,L-6和SS升高(P0.05,P0.01);②与单纯脾虚组相比,脾虚发热组大鼠体温在30min时有一过性降低,之后体温一直呈显著性升高(P0.01);外周血T4含量显著性降低(P0.01),其余各指标的差异无显著性。结论:饮食失节+过度疲劳+LPS注射可引起大鼠出现类中医脾虚发热证。

Research on the rat model of fever due to spleen deficiency syndrome

Objective:To copy and value the model of spleen-qi deficiency with fever.Methods:The rats were assigned into the normal group,spleen-deficiency group,spleen-deficiency with fever group.The rats were treated with excess fatigue-out of constant diet to copy spleen-qi deficiency rat model.On the 22th day,The rats of spleen-qi deficiency with fever group were respectively ip with LPS(80μg/kg)to induce fever.The rectal temperature was measured.The body weight and change of syndrome and autonomous behavior was observed.D-xylose of urine and peripheral blood GAS,MTL,SS,IL-1β,IL-2,IL-6,T3,T4,TSH are measured.Result:①Compared with the normal group,the rectal temperature of spleen-qi deficiency with fever group has a transient decrease at 30min,then raised obviously(P<0.05,P<0.01);the spleen-qi deficiency group has a transient decrease at 60min,then return to the basic line.The body weight of spleen-qi deficiency and spleen-qi deficiency with fever group were decreased obviously at the end of 3w(P<0.01) and the integral quantity of appearance behavior change were significantly increased at the weekend of 2w and 3w(P<0.05,P<0.01);Urine D-xylose excretion rate,peripheral blood GAS,MTL,IL-1β,IL-2,T3,T4,TSH decreased and IL-6,SS increased(P<0.05,P<0.01).②Compared with the spleen-qi deficiency group,temperature of spleen-qi deficiency with fever group has a transient decrease at 30min,then raised obviously(P<0.01).Blood T4 has decreased obviously(P<0.01),other indexes have no significance.Conclusion: By adopting excess fatigue +out of constant diet +LPS injection had induced the rats show a serial of appearance in syndrome model of autonomous behavior,body weight,body temperature which were similar to Chinese symptoms of spleen-qi deficiency with fever.