BRCA1和p120ctn在乳腺浸润性导管癌中的表达及其临床意义

目的:观察乳腺浸润性导管癌组织中BRCA1和p120ctn表达及其与临床病理特征之间的关系,探讨它们在乳腺浸润性导管癌发生、发展中的作用及临床应用价值。方法:采用免疫组化方法,检测BRCA1蛋白及p120ctn在乳腺浸润性导管癌、乳腺腺病和癌旁正常组织的表达。分析两者的表达与各病理特征的关系及其两者的相关性。结果:在60例乳腺浸润性导管中,BRCA1蛋白及p120ctn阳性率分别为60.0%(36/60)和35.0%(21/60),分别低于各自在乳腺腺病和癌旁正常组织中的表达(均P<0.05);BRCA1蛋白表达状态与患者高发病年龄、临床病理分期、伴有腋淋巴结转移以及组织学分级有关(均P<0.05),而与肿瘤大小无关(P>0.05);p120ctn表达状态与临床病理分期、伴有腋淋巴结转移以及组织学分级有关(均P<0.05),而与发病年龄、肿瘤大小无关(均P>0.05);BRCA1蛋白与p120ctn在乳腺浸润性导管癌组织中的表达呈正相关(r=0.314,P<0.05)。结论:BRCA1与p120ctn的联合检测可作为乳腺浸润性导管癌诊断、恶性程度及预后判断的指标,为临床个体化治疗提供依据。

Expression of BRCA1 and p120-catenin in invasive ductal carcinoma of breast and its clinical significance

Objective:To investigate the expressions of breast cancer susceptibility gene 1(BRCA1) protein and p120-catenin(p120ctn) in invasive ductal carcinoma(IDC) of the breast and their relations with the clinicopathologic characteristics of this tumor,so as to determine their roles in the occurrence and development of IDC and their clinical application values. Methods:The expression of BRCA1 protein and p120ctn in the specimens of IDC,breast adenosis and tumor adjacent breast tissue were detected by immunohistochemical method.The relations of both expressions with the various clinicopathologic characteristics of IDC and correlation between the both were analyzed. Results:Among the 60 cases of IDC specimens,the positive expression rates of BRCA1 protein and P120ctn were 60.0%(36/60) and 35.0%(21/60),and both expressions were significantly lower than those in the breast adenosis and tumor adjacent breast tissues,respectively(all P<0.05).The expression status of BRCA1 protein was related to high incidence age,clinical stage,presence of axillary lymph node metastases and histological grade of the patients(all P<0.05),but unrelated to their tumor size(P>0.05).The expression status of p120ctn was relevant to the clinical stage,presence of axillary lymph node metastases and histological grade of the patients(all P<0.05),but irrelevant to their age at onset of disease and tumor size(both P>0.05).There was a positive correlation between the expression of BRCA1 protein and p120ctn in IDC tissues(r=0.314,P<0.05). Conclusion:The combination detection of BRCA1 protein and p120ctn can be used for diagnosis and determination of the degree of malignancy and prognosis of IDC,and provide a basis for the individualized treatment of IDC patients.