| 尖锐湿疣组织HPV基因芯片分型检测在临床中的应用 |
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| 引用本文： | 陈雷,赵敏,张小林. 尖锐湿疣组织HPV基因芯片分型检测在临床中的应用[J]. 标记免疫分析与临床, 2012, 19(4): 213-216 |
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| 作者姓名： | 陈雷 赵敏 张小林 |
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| 作者单位： | 武汉市皮肤病防治研究所,湖北武汉,430031;武汉市皮肤病防治研究所,湖北武汉,430031;武汉市皮肤病防治研究所,湖北武汉,430031 |
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| 摘 要： | 目的 探讨一种适合于临床应用的HPV- DNA分型检测方法.方法 利用HPV保守区引物PCR扩增和型特异性探针膜芯片杂交,对包括5种低危型( HPV6、11、42、43、44)和18种高危型(HPV16、18、31、33、35、39、45、51、52、53、56、58、59、66、68、73、83、MM4) HPV进行...
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| 关 键 词： | 尖锐湿疣 基因芯片 HPV |
Clinical Application of the HPV Genotype of Condyloma Acuminata Tissues with Gene-chips |
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| CHEN Lei , ZHAO Min , ZHANG Xiao-lin. Clinical Application of the HPV Genotype of Condyloma Acuminata Tissues with Gene-chips[J]. Labeled Immunoassays and Clinical Medicine, 2012, 19(4): 213-216 |
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| Authors: | CHEN Lei ZHAO Min ZHANG Xiao-lin |
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| Affiliation: | (Wuhan institute of Dermatology and Venereology,Wuhan 430031,China) |
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| Abstract: | Objective To explore a clinical method for detection of HPV-DNA genotype in condyloma acuminate（CA） with gene-chips.Methods The gene-chips technique was used to determine 23 types which include 5 low risk and 18 high risk of HPV genotype in tissues from 281 patients with CA.Results There were 265 positive cases in 281 patients with CA,the total positive rates was 94.31%.The positive rates of low risk HPV genotypes was 84.35% with 224 cases,and high risk HPV genotypes was 15.47% with 41 cases.The positive rate from single infection with HPV was 84.15% with 223 cases and the mixed infection with HPV was 15.85% with 42 cases.Conclusion Gene-chips method is high sensitivities and specificities method in detection and typing of HPV.It is suitable for clinical diagnostic and research of HPV. |
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| Keywords: | Condyloma acuminata Gene-chips Human papillomavirus |
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