| 自噬基因Beclin-1和微管相关蛋白LC3在大鼠阿霉素心肌病中的表达及其意义 |
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| 引用本文： | 黄洁,严文华,吕海涛,孙凌,徐秋琴.自噬基因Beclin-1和微管相关蛋白LC3在大鼠阿霉素心肌病中的表达及其意义[J].临床儿科杂志,2010,28(1). |
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| 作者姓名： | 黄洁 严文华 吕海涛 孙凌 徐秋琴 |
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| 作者单位： | 苏州大学附属儿童医院,江苏苏州,215003 |
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| 摘 要： | 目的探讨自噬基因Beclin-1及微管相关蛋白LC3在大鼠阿霉素心肌病中的表达及其意义,初步证实自体吞噬(autophagy)参与了大鼠阿霉素心肌病的发病,并探讨其机制。方法雄性SD大鼠45只,随机分为3组,阿霉素(ADR)组、阿霉素(ADR)+3-甲基腺嘌呤(3-MA)组和对照组。建立大鼠阿霉素心肌病模型,电镜观察自噬体形态及数量,检测心肌组织中Beclin-1含量及LC3含量。结果ADR组大鼠心肌组织自噬体的数量较对照组及ADR+3-MA组增多;ADR组心肌组织中Beclin-1水平及LC3含量均较对照组及ADR+3-MA组增高。结论自噬性程序性细胞死亡参与了阿霉素心肌病的发病。
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| 关 键 词： | 阿霉素 心肌病 Beclin-1 微管相关蛋白LC3 3-甲基腺嘌呤 自体吞噬 |
Expression of Beelin-1 and LC3 in adriamycin induced cardiomyopathy rats |
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| HUANG Jie,YAN Wen-hua,LU Hai-tao,SUN Ling,XU Qiu-qin.Expression of Beelin-1 and LC3 in adriamycin induced cardiomyopathy rats[J].The Journal of Clinical Pediatrics,2010,28(1). |
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| Authors: | HUANG Jie YAN Wen-hua LU Hai-tao SUN Ling XU Qiu-qin |
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| Institution: | Children's Hospital Affiliated to Soochow University/a>;Suzhou 215003/a>;Jiangsu/a>;China |
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| Abstract: | Objective To explore the expressions of autophagy-related gene Beclin-1 and LC3 in adriamycin induced cardiomyopathy rats,to prove that autophagy might take part in the development of adriamycin induced cardiomyopathy in rats,so as to provide experimental and theoretical evidence for preventing and treating adriamycin induced cardiomyopathy. Methods Forty-five male SD rats were randomly divided into 3 groups,control groups,ADR group and ADR+3-MA group. The model of adriamycin induced cardiomyopathy rats was... |
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| Keywords: | adriamycin cardiomyopathy Beclin-1 LC3 3-MA autophagy |
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