| 反义寡核苷酸药代动力学研究中的定量分析技术 |
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| 引用本文： | 鲁丹丹,王升启. 反义寡核苷酸药代动力学研究中的定量分析技术[J]. 国际药学研究杂志, 2007, 34(1): 17-20 |
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| 作者姓名： | 鲁丹丹 王升启 |
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| 作者单位： | 军事医学科学院放射与辐射医学研究所,北京,100850;军事医学科学院放射与辐射医学研究所,北京,100850 |
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| 摘 要： | 反义寡核苷酸药物的药代动力学研究要求具备相应的生物定量方法。本文就近年来在临床前和临床药代动力学评价中常用的几种定量方法，包括放射性同位素法、毛细管凝胶电泳法、高效液相色谱法、液质联用和基于杂交技术的酶联免疫法，对其各自的特性、应用前景、操作方法及局限性等方面进行了综述。
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| 关 键 词： | 反义寡核苷酸 生物定量方法 药代动力学 |
| 文章编号： | 1001-0971(2007)01-0017-04 |
| 收稿时间： | 2006-06-22 |
| 修稿时间： | 2006-06-22 |
Quantitative bioanalytical methods for pharmacokinetics of antisense oligonucleotides |
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| LU Dan-dan,WANG Sheng-qi. Quantitative bioanalytical methods for pharmacokinetics of antisense oligonucleotides[J]. Foreign Medical Sciences(Section of Pharmarcy), 2007, 34(1): 17-20 |
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| Authors: | LU Dan-dan WANG Sheng-qi |
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| Affiliation: | (Department of Biotechnology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China) |
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| Abstract: | The pharmacokinetic researches of antisense therapeutic agents require the development of a number of bioanalytical methods for their quantitation Recently, the most common bioanalytical techniques used in preclinical and clinical pharmacokinetic assessment include radiotracer methods, CGE with UV detection or with LIF detection, HPLC, LC MS/MS, and hybridization based ELISA Their specific features, application prospects, characteristics of operation and limitations are summarized in this review. |
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| Keywords: | antisense oligonucleotide bioanalytical method pharmacokinetics |
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