| MAGE-1、MAGE-3基因在胃癌和胃活检组织中的表达及意义 |
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| 引用本文： | 陈雪华,刘炳亚,张冬青,张奕,张轶,李建芳,朱正纲. MAGE-1、MAGE-3基因在胃癌和胃活检组织中的表达及意义[J]. 细胞与分子免疫学杂志, 2004, 20(3): 310-313 |
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| 作者姓名： | 陈雪华 刘炳亚 张冬青 张奕 张轶 李建芳 朱正纲 |
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| 作者单位： | 1. 上海第二医科大学附属瑞金医院外科,上海消化外科研究所
2. 上海市免疫学研究所,上海,200025 |
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| 基金项目： | 国家自然科学基金资助项目 (No .30 1 70 91 5) |
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| 摘 要： | 目的 检测黑色素瘤抗原基因-1、 -3(MAGE-1和MAGE-3)在胃癌中的表达, 探索其在胃癌免疫治疗中的应用价值.方法 采用RT-PCR法, 对36例胃癌患者的癌组织和相应的癌旁"正常"黏膜, 以及37例慢性胃炎患者的胃镜活检组织中, MAGE-1和MAGE-3基因的表达进行研究, 并对RT-PCR扩增产物中的目的基因片段进行DNA测序验证.结果 36例胃癌组织中, MAGE-1基因的表达阳性率为72.22%(26/36), MAGE-3基因的表达阳性率为69.44%(25/36).MAGE-1、 MAGE-3的表达均为阳性者为52.78%(19/36).癌旁"正常"黏膜中, MAGE-1、 MAGE-3基因的表达阳性率分别为47.22%(17/36)和44.44%(16/36); MAGE-1、 MAGE-3基因表达双阳性者为30.55%(11/36).在37例胃慢性炎症患者中, MAGE-1、 MAGE-3基因的表达阳性率, 分别为29.73%(11/37)和27.03%(10/37); MAGE-1、 MAGE-3基因的表达双阳性者为8.1%(3/37).胃癌组织中, MAGE-1和MAGE-3基因表达阳性率均高于癌旁和胃慢性炎症患者; 在肿瘤组织中的表达阳性率与肿瘤浸润的深度和分化程度有关(P<0.05); 而与临床病理分期及淋巴结转移无关(P>0.05).结论 基于MAGE-1和MAGE-3基因在胃癌中的高表达率, 可利用这两种蛋白作为靶分子进行免疫治疗, 同时也可作为一种临床有用的随访指标.
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| 关 键 词： | 胃癌 慢性胃炎 MAGE-1 MAGE-3 基因表达 |
| 文章编号： | 1007-8738(2004)03-0310-04 |
| 修稿时间： | 2003-07-16 |
Expression of MAGE-1 and MAGE-3 genes in gastric cancer and gastric biopsy tissues and its clinical significance |
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| CHEN Xue hua ,LIU Bing ya ,ZHANG Dong qing ,ZHANG Yi ,ZHANG Yi ,LI Jian fang ,ZHU Zheng gang. Expression of MAGE-1 and MAGE-3 genes in gastric cancer and gastric biopsy tissues and its clinical significance[J]. Chinese journal of cellular and molecular immunology, 2004, 20(3): 310-313 |
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| Authors: | CHEN Xue hua LIU Bing ya ZHANG Dong qing ZHANG Yi ZHANG Yi LI Jian fang ZHU Zheng gang |
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| Affiliation: | Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China. xhchensh@yahoo.com |
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| Abstract: | AIM: To detect the expression of melanoma antigen 1 and 3 genes (MAGE 1 and MAGE 3) and explore its significance in immunotherapatic application. METHODS: The expression of MAGE 1 and MAGE 3 in gastric cancer, para tumorous normal mucosa, and gastric biopsy tissues were detected by RT PCR. The target genes in RT PCR product were verified by means of DNA sequencing. RESULTS: MAGE 1 expression was positive in 26 out of 36 (72.22%) cases of gastric cancer, MAGE 3 positive in 25 out of 36(69.44%), and both MAGE 1 and MAGE 3 positive in 19 out of 36 (52.78%). Positive expression rates of MAGE 1, MAGE 3 and both MAGE 1 and MAGE 3 were 47.22%, 44.44% and 30.55%, respectively in para tumorous normal mucosa, while 29.73%, 27.03% and 8.1% in gastric biopsy tissues The positive rates of MAGE 1 and MAGE 3 gene expressions in tumor tissues was significant higher than those its parallelism mucosae and biopsy tissues from chronic gastritis. The expressions of MAGE 1 and MAGE 3 genes in tumor tissues were relevant to intruded depth and the differentiated degree of tumor cells ( P <0.05). CONCLUSION: MAGE 1 and MAGE 3 protein may be used as targets molecules of immunotherapy for gastric cancer and indexes for follow up study of chronic gastritis as well. |
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| Keywords: | stomach cancer chronic gastritis MAGE 1 MAGE 3 gene expression |
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