咖啡酸苯乙酯对人大肠癌HCT116细胞裸鼠皮下移植瘤生长的影响

目的观察咖啡酸苯乙酯（CAPE）对人大肠癌裸鼠皮下移植瘤生长的影响。方法以大肠癌细胞株HCT116为研究对象，对20只BAL13／c裸鼠建立人大肠癌皮下移植瘤模型，随机分为对照组和治疗组各10只。治疗组以CAPE5mg／d，观察两组第7、14、21、28天皮下移植瘤生长情况及裸鼠体重的变化；治疗结束时取肿瘤组织及心、肝、肺、肾、肠等行病理组织学检查，TUNEL法检测肿瘤细胞凋亡。结果CAPE对HCT116裸鼠移植瘤的生长有明显抑制作用，与对照组相比，瘤体重量明显降低、体积减小（P〈0．01），坏死面积显著增加，凋亡指数明显升高，但对裸鼠体重无明显影响，心、肝、肺、肾、肠等组织未见明显病理学改变。结论CAPE具有抑制人大肠癌裸鼠皮下移植瘤生长的作用，可能与其诱导细胞凋亡有关。

Effect of caffeic acid phenethyl ester on the growth of human colorectal carcinoma cell line HCT116 transplanted subcutaneously in nude mice

Objective To investigate the effect of caffeic acid phenethyl ester (CAPE) on the growth of human colorectal carcinoma cell line HCT116 transplanted subcutaneously in nude mice. Methods Nude mouse model of human colorectal carcinoma by subcutaneous transplantation of HCT116 cell line was reproduced. A total of 20 mice were divided into 2 groups: control group and CAPE group (oral administration of CAPE at 5mg/mice/d). The growth of the subcutaneously transplanted tumor and changes in mouse body weight in each group after treatment were observed on 7, 14, 21 and 28d. Histopathological examination of xenograft, heart, liver, lung, kidney and intestine of nude mice was also conducted. Apoptosis index was detected by terminal dUTP nick end labeling (TUNEL) technique. Results CAPE had significantly inhibitory effect on growth of the transplanted xenograft in vivo. Tumor volume and tumor weight were decreased (P<0.01). The size of necrotic areas was found to be enlarged significantly in CAPE-treated group. CAPE had not significant effect on the weight of nude mice, nor induction of significant histopathological change in the heart, liver, lung, kidney and intestine of nude mice. Conclusion CAPE has inhibitory effect on the growth of human colorectal carcinoma transplanted subcutaneously in nude mice. The results provide the evidence for the potential usefulness of CAPE for the prevention and treatment of human colorectal carcinoma.