党参对大鼠脑缺血再灌注损伤的保护作用

目的 研究党参(Codonopsis pilosula．)对大鼠脑缺血再灌注损伤的保护作用，并从改善能量代谢角度探讨其作用机制。方法 Wistar大鼠随机分为假手术组，缺血再灌注 生理盐水组，缺血再灌注 党参组，缺血再灌注 CoQ10组。其中缺血再灌注 生理盐水组、缺血再灌注 党参组、缺血再灌注 CoQ10组分别连续5d灌胃口服生理盐水、党参浸提液、CoQ10混悬液。采用阻断大鼠双侧颈总动脉和尾部放血，并重复缺血再灌注的脑缺血模型，分别于缺血后3，6，12h断头取脑，采用高效液相法测定脑组织中ATP含量、比色法测定Na^ ，K^ —ATPase活性。结果 生理盐水对照组中大鼠脑组织ATP含量、Na^ ，K^ —ATPase活性明显低于假手术组(P＜0．05)，党参组、CoQ10组中大鼠脑组织ATP含量、Na^ ，K^ —ATPase活性明显高于生理盐水对照组(P＜0．05)，且二者间无显著性差异。结论 党参可改善缺血再灌注脑细胞能量代谢，具有脑保护作用，作用效果与CoQ10相近。

Protective effect of Codonopsis pilosula on ischemia and reperfusion-induced brain injury in Wistar rats

Objective To investigate the protective effect of Codonopsis pilosula on ischemia and reperfusion-induced brain injury in Wistar rats and the mechanism of its action.Methods One hundred Wistar rats were divided randomly into 4 groups: Group A, sham-operated(SH); Group B, cerebral ischemia and reperfusion+NS(IR+NS); Group C, cerebral ischemia and reperfusion+Codonopsis pilosula (IR+Codo.); Group D, cerebral ischemia and reperfusion+CoQ10 (IR+CoQ10). Group B,C,D,were feed with NS,Codonopsis pilosula,CoQ10 respectively .The common cervical arteries of the rats were transient blocked bilaterally, in association with bleeding from their tails and followed by reperfusion. The procedures were repeated once more again as above to establish a stable ischemic brain injury model. The levels of ATP, Na+, K +ATPase activity in brain tissue were determinated after 3,6,12h from the beginning of ischemia. Results The level of ATP and Na+,K +ATPase activity of Group B(IR+NS) were lower than those of Group A(SH)(P<0.05) and the level of ATP and Na+,K+ATPase activity of Group C(IR+Codo.) and Group D( IR+CoQ10)were both higher than those of GroupB(IR+NS)(P<0.05).There was no difference between the values of Group C and Group D.Conclusions Codonopsis pilosula could improve the energy metabolism of brain and had the protective effect on brain. It's effect is approach to that of CoQ10.