复方壳多糖对生长期大鼠体质量和血脂水平的影响

背景实验和临床研究证实壳多糖具有减肥和降低血脂的作用,但是因用量较大存在一定的副作用.目的观察复方壳多糖对生长期大鼠体质量和血脂水平的影响.设计完全随机化分组实验.地点和材料80只 SPF级别的 3～ 4周龄健康雄性 SD大鼠分为 4组饲养于四川省卫生管理干部学院具有屏障系统的恒温动物房内,各实验指标检测在本院实验室进行.干预A组为正常对照组,喂饲基础饲料、 B组为高脂对照组,喂饲高脂饲料、C组为壳多糖组,喂饲壳多糖饲料、 D组为复方壳多糖组,喂饲复方壳多糖.主要观察指标饲养4周后检测各组动物体质量等生长发育指标和血清脂质.结果①体长和尾长各组动物饲后均有不同程度的增加,尤其是C组 [(17.73± 0.99)cm]明显,与 A组 [(16.50± 1.03)cm]和 B组 [(16.35± 0.74)cm]差异有显著性意义(P《 0.05).②三酰甘油 B组 [(1.5680± 0.3000)mmol/L]显著高于 A[(0.9404±0.2864)mmol/L]、 D[(0.6665± 0.2567)mmol/L]和 C组 [(0.8629± 0.3382)mmol/L]( P《0.05); D组最低,与 A、 C组差异也有显著性意义( P《 0.05).③ HDL-c和 HDL-c/三酰甘油D组 [分别为( 0.6367± 0.1223) mmol/L和 0.265]明显增高,与 B组 [分别为( 0.4821±0.1058) mmol/L和 0.200]、 C组 [分别为( 0.5213± 0.0938) mmol/L和 0.209〗比较差异有显著性意义(P《 0.05).结论一定量的壳多糖摄入对生长期动物发育无影响,同时使三酰甘油水平降低,复方壳多糖减肥降脂作用明显优于单纯使用壳多糖,并使HDL-c升高.

Effect of compound chitosan on body mass and blood lipid level of rats in growing period

BACKGROUND:Experimental and clinical studies have demonstrated that chitosan can reduce body mass and blood lipid level.However,it induces some side effects due to large doses. OBJECTIVE:To explore the effect of compound chitosan on body mass and blood lipid level of rats in growing period. DESIGN:Completely randomized grouping experiment. SETTING AND PARTICIPANTS:A total of 80 healthy SD rats(SPF degree),aged 3- 4 weeks old,were divided into 4 groups and fed in the animal room with constant temperature with barrier system in Sichuan Continuing Education College of Medical Sciences.All experimental indexes were measured in the laboratory of our college. INTERVENTIONS:Group A was normal control group and treated with essential diet;Group B was high lipid control group and treated with high lipid diet;Group C was chitosan group and fed with chitosan diet;Group D was compound chitosan group and treated with compound chitosan diet. MAIN OUTCOME MEASURES:Growth and development indexes like body mass and blood serum lipid of the rats four weeks after feeding. CONCLUSION:A certain amount of chitosan intake has no effect on the growth of rats in growing period,and it can lower serum TG level.Compound chitosan has greater effects of both reducing serum lipids and body mass and raising HDL-C level than chitosan alone.