吸烟对肺泡巨噬细胞凋亡的影响

目的：观察吸烟或不吸烟者肺泡巨噬细胞凋亡的变化。方法：对吸烟或不吸烟者肺泡巨噬细胞进行染色观察及流式细胞仪ＤＮＡ分析。结果：吸烟者肺泡巨噬细胞生存时间延长，吸烟组存活率为（７６．３±１２．５）％，不吸烟组为（５５．４±１１．６）％，两者差异显著（Ｐ＜０．０５）。荧光染色吸烟组凋亡细胞百分率为（３８．６±１１．５）％，不吸烟组凋亡细胞百分率为（５９．４±１２．１）％。流式细胞仪观察凋亡细胞百分率吸烟组为（１１．５±１０．４）％，不吸烟组为（２９．１±８．６）％。吸烟组巨噬细胞凋亡较不吸烟者明显减少。结论：烟雾中某些成分可能通过抑制巨噬细胞凋亡，在肺气肿的发病中起重要作用。

Influences of cigarette smoking on the apoptosis of alveolar macrophages

Objective: To study the difference of alveolar macrophage apoptosis between smokers and nonsmokers. Methods: Alveolar macrophages was obtained through alveolar lavage from 8 smokers and 8 nonsmokers. The alveolar macrophages was cultured and stained by trypan blue dye and fluorescence dye. Flow cytometry was used to analyze apoptosis rate of alveolar macrophages. Results: The survival rate of smoker group (76. 3 * 12, 5 ) % was higher than that of nonsmoker group (55. 4 * 11. 6) %,the apoptic rate was lower in smoker group (38. 6* 11. 5) % than in nonsmoker group (59. 4* 12. 1 ) %. Flowcytometer test revealed apoptic rate in smoker group was (11. 5 * 10. 4 ) %, while nonsmoker group was (29. 1 * 8. 6 ) %. Conclusion:Cigarette smoking can cause abnormal apoptosis of alveolar macrophages,which may play an important role in emphysema formation.