苯那普利对糖尿病大鼠心肌CTGF基因表达的影响及作用机制

目的：研究苯那普利对结缔组织生长因子（CTGF）mRNA在糖尿病（DM）大鼠心肌中表达的影响及相关机制。方法：用24只Wistar大鼠建立DM模型后，随机分为苯那普利治疗组及DM未治疗组，处理12周末检测血糖、血脂，应用放免法检测心肌血管紧张素Ⅱ（AngⅡ）的含量，应用RT-PCR检测心肌CTGF mRNA表达，并与正常组对照。结果：DM未治疗组血糖、血脂明显增高，心肌AngⅡ含量及CTGR mRNA表达明显高于正常组和苯那普利治疗组（P<0.01）。结论：持续血糖增高能导致大鼠心肌AngⅡ含量增加和心肌CTGF mRNA表达增加，苯那普利能在一定程度上抑制大鼠心肌CTGF mRNA表达，其机制可能与其减少心肌AngⅡ含量有关。

Effects of benazepril on CTGF in myocardium of diabetic rats and its mechanism

Objective To investigate the effects of benazepril on connective tissue growth factor(CTGF)expression in myocardial tissue of streptozotocin(STZ)-induced experimental diabetic rats and its mechanism. Methods A total of 34 Wistar rats were enrolled in the study, 10 of them were set as normal controls, others were used to set up the diabetes mellitus models by injecting STZ, and then equally divided into benazepril-treated group and untreated group. Blood glucose and Tcho levels levels were determined, myocardial AngⅡ was detected by immunoradiometry and myocardial CTGF mRNA was assayed by RT-PCR after 12 weeks. Results Blood glucose and Tcho increased significantly in untreated group, the levels of myocardial AngⅡ and CTGF mRNA expression were higher than those in normal control and benazepril-treated group (P<0.01). Conclusion Long lasted hyperglycemia can lead to the over-expression of CTGF mRNA in the myocardial tissue of diabetic rats. Benazepril may partly suppress it and decrease significantly the myocardial injury of diabetic rats by reducing the level of myocardial AngⅡ.