Elevated granzyme M-expressing lymphocytes during cytomegalovirus latency and reactivation after allogeneic stem cell transplantation |
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Authors: | Niels Bovenschen Sanne NM Spijkers Annette C Wensink Ingrid MM Schellens Robert van Domselaar Debbie van Baarle |
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Institution: | 1. Department of Pathology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands;2. Laboratory for Translational Immunology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands;3. Department of Internal Medicine and Infectious Diseases, University Medical Center Utrecht, 3584 EA Utrecht, The Netherlands |
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Abstract: | Human cytomegalovirus (HCMV) reactivation can cause serious complications in allogeneic stem cell transplantation (SCT) patients. HCMV is controlled by cytotoxic lymphocytes that release antiviral granzymes. Recently, we have demonstrated that granzyme M (GrM) inhibits HCMV replication in vitro, however the physiological role of GrM and its cellular distribution during HCMV infection remains unknown. Here, we examined GrM expression in lymphocyte populations during HCMV infection. The percentage of GrM-expressing effector-memory CD4+ T-cells was higher in HCMV latently-infected healthy individuals compared to that of uninfected individuals. SCT recipients had higher percentages of GrM-expressing CD4+ T, CD8+ T, γδT, and NKT cells. Despite lower total T-cell numbers, HCMV reactivation in SCT patients specifically associated with higher percentages of GrM-expressing CD4+ (total and central-memory) T-cells. GrM was elevated in plasma during HCMV reactivation, pointing to extracellular perforin-independent functions of GrM. We conclude that GrM may be important in regulating HCMV latency and reactivation in SCT patients. |
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Keywords: | HCMV human cytomegalovirus SCT stem cell transplantation GrM granzyme M GrB granzyme B |
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