人眼视网膜母细胞瘤细胞凋亡的研究

目的 观察人视网膜母细胞（ｒｅｔｉｎｏｂｌａｓｔｏｍａ，ＲＢ）标本中细胞凋亡，探讨细胞凋亡在ＲＢ发生发展及消退中的作用。方法 用ＴＵＮＥＬ方法光镜和电镜观察人ＲＢ摘除眼球标本中是否存在细胞凋亡。结果 １５例人ＲＢ分化型和未分化型标本中有９例发现少量凋亡细胞，自发退化型ＲＢ中未发现凋亡细胞。每例中均见成片出现的坏死细胞。结论 视网膜母细胞瘤细胞死亡通过坏死与凋亡２种途径。细胞凋亡是ＲＢ自发退化的机制

Studies on apoptosis in human retinoblastoma

Objective To determine the frequency of apoptosis and to investigate the effect of apoptosis on the generating and developing of RB.Methods Seventeen human RB specimens were selected, including 7 undifferentiated, 8 differentiated, and 2 spontaneous regression tumor specimens. Apoptotic cells were identified with the transferase(TDT) mediated duTP biotin nick end labeling(TUNEL) method and electron microscopy.Results A few apoptotic cells were found in 9 of 15 human retinoblastoma specimens but no apoptotic cells were detected in 2 spontaneous regression RB specimens. Conclusion Necrosis and apoptosis are the two major ways of RB cell dying. There is no sufficient evidence that the cell apoptosis is the mechanism of spontaneous regression of RB.