普伐他汀提升肾病综合征合并高凝状态大鼠抗凝血酶Ⅲ水平

目的观察并比较普伐他汀和低分子肝素对肾病综合征高凝状态的影响.方法经尾静脉一次性注入阿霉素(2%的阿霉素,6.5 mg·kg-1)制作肾病综合征模型,观察普伐他汀、低分子肝素及联合用药对血浆凝血与纤溶分子标志物抗凝血酶Ⅲ、D-二聚体和纤维蛋白原的影响.结果普伐他汀和低分子肝素均可使血浆D-二聚体含量下降,普伐他汀可使肾病模型大鼠的抗凝血酶Ⅲ水平明显升高,而低分子肝素组抗凝血酶Ⅲ水平与模型组差异无统计学意义,三个治疗组的纤维蛋白原浓度、血液生化指标与模型组差异均没有统计学意义.结论阿霉素诱导的大鼠肾病综合征可出现明显的高凝状态,普伐他汀明显升高血浆ATⅢ水平,改善肾病综合征大鼠高凝状态.

Pravastatin improves level of antithrombin Ⅲ in rats combined nephrotic syndrome with hypercoagulability

AIM: To investigate the anticoagulant effect of pravastatin and low molecular weight heparin (LMWH), as well as their combination, over time, in a rat model of experimental nephrotic syndrome. METHODS: Healthy SD male rats were chosen randomly to perform nephrotic syndrome models by single injection of adriamycin via tail vein, the matched normal control rats received single injection of equivalent 0.9% sodium chloride instead. After 14 days, the models were set up and randomized into model control group, pravastatin group (pravastatin 2 mg·kg-1·d-1 gavage once a day), LMWH group(LMWH 200 U·kg-1·d-1 intraperitoneal injection once a day) and combined treatment group(pravastatin 2 mg·kg-1·d-1 gavage+ LMWH 200 μ·kg-1·d-1 intraperitoneal injection), then all rats underwent measuring proteinuria every two weeks and fibrinogen, antithrombinⅢ(ATⅢ), D-dimer, platelet count, serum total protein and serum albumin after 4 weeks of treatment. RESULTS: The concentration of fibrinogen and D-dimer in model group was higher significantly than that in control group, and the level of ATⅢ was lower remarkably than that in control group, but platelet count had no obvious change; Compared with model group, pravastatin could increase the level of ATⅢ and decrease the concentration of D-dimer, but the concentration of fibrinogen and platelet count did not change obviously; LMWH and combined treatment could also decrease level of D-dimer, but had no great effects on ATⅢ, fibrinogen and platelet count; all treatment group had no obvious change of serum total protein and serum albumin. CONCLUSION: Adriamycin-induced nephrotic syndrome rat models have hypercoagulability and pravastatin can increase the level of ATⅢ.