| 多重PCR诊断真菌性鼻-鼻窦炎的实验研究 |
| |
| 作者姓名： | Zhang Z Xu M Zhang J |
| |
| 作者单位： | 南昌大学第一附属医院耳鼻咽喉头颈外科,南昌,330006 |
| |
| 摘 要： | 目的:构建一多重PCR反应体系,并探讨其对真菌性鼻-鼻窦炎(FRS)快速诊断和同时鉴定常见致病菌的临床应用.方法:选择3对引物(真菌通用引物、曲霉特异性引物、毛霉特异性引物)建立多重PCR反应体系,采用曲霉菌、毛霉菌、链格孢菌、白色念珠菌等标准菌株DNA验证体系的适用性和摸索反应条件,同时将多重PCR、真菌培养2种方法用于FRS的鼻窦内容物的真菌检测,比较二者敏感性.结果:3对引物具有较好的通用性和特异性,在适宜的反应条件下均能扩增出目的片段.该反应体系的检测灵敏度为10 μg/L.20例FRS标本,真菌培养检测阳性7例,阳性率35%;多重PCR体系检测阳性15例,阳性率75%,两者差异有统计学意义(P<0.05).结论:该多重PCR体系是一种快速、敏感和特异性诊断方法,可对FRS做出快速诊断并鉴定常见的致病菌属.
|
| 关 键 词： | 真菌 鼻窦炎 聚合酶链反应 |
Experimental and clinical study on the diagnosis for pathogenic fungi of fungal rhinosinusitis by multiplex PCR |
| |
| Zhang Z,Xu M,Zhang J.Experimental and clinical study on the diagnosis for pathogenic fungi of fungal rhinosinusitis by multiplex PCR[J].Journal of Clinical Otorhinolaryngology,2011,25(8):367-370. |
| |
| Authors: | Zhang Zhiyuan Xu Meiling Zhang Jian |
| |
| Institution: | ZHANG Zhiyuan XU Meiling ZHANG Jian (Department of Otolaryngology Head and Neck Surgery,the First Affiliated Hospital of Nanchang University,Nanchang,330006,China) |
| |
| Abstract: | Objective:To develop a multiplex PCR method used to fast diagnose pathogenic fungi of fungal rhinosinusitis.Method:Three pairs of primers(universal fungus-specific primers,aspergillus specific primers,mucor specific primers)were used to establish multiplex PCR system.Detected six important medical fungi species strains to examine the applicability of the system and explore the optimum conditions.Multiplex PCR system was also used to identify pathogenic fungi of fungal rhinosinusitis,and used to comparethe s... |
| |
| Keywords: | fungus rhinosinusitis polymerase chain reaction |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
|
