| 重组人促红细胞生成素后处理对兔缺血／再灌注骨骼肌细胞凋亡及超微结构的影响 |
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| 引用本文： | 张谦,耿喜林,杨同群,李惠萍,康迎新. 重组人促红细胞生成素后处理对兔缺血／再灌注骨骼肌细胞凋亡及超微结构的影响[J]. 兰州大学学报(医学版), 2011, 37(1): 35-38,43 |
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| 作者姓名： | 张谦 耿喜林 杨同群 李惠萍 康迎新 |
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| 作者单位： | 张谦,耿喜林,杨同群,康迎新,ZHANG Qian,GENG Xi-lin,YANG Tong-qun,KANG Ying-xin(兰州大学第二医院急救中心,甘肃,兰州,730030);李惠萍,LI Hui-ping(甘肃省紧急医疗救援中心医教科,甘肃,兰州,730030) |
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| 摘 要： | 目的探讨重组人促红细胞生成素（rhEPO）后处理对家兔缺血／再灌注（I／R）骨骼肌细胞凋亡及超微结构的影响。方法将24只成年家兔随机分为对照绀、I／R组和rhEPO后处理组（5000IU／kg）,采用25％的乌拉坦4mL／kg耳缘静脉注射麻醉,在I／R组、rhEPO后处理组建立兔左后肢腓肠肌I／R实验模型,4h后松开动脉夹,恢复血流灌注,12h后切取腓肠肌标本,采用原位末端标记法检测3组细胞凋亡指数,对I／R早期骨骼肌病理标本进行光镜及电镜观察。结果rhEPO后处理组细胞凋亡较I／R组明显减少,差异有统计学意义（P〈0．05）;电镜及光镜下腓肠肌组织病理改变显示,rhEPO后处理组病变程度明显轻于I／R组。结论rhEPO后处理可通过减少细胞凋亡,改善骨骼肌I／R损伤。
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| 关 键 词： | 重组人促红细胞生成素 后处理 兔缺血／再灌注损伤 骨骼肌 细胞凋亡 超微结构 |
Effects of recombinant human erythropoietin postconditioning on apoptosis and ultrastructure from ischemia/reperfusion injury in rabbits skeletal muscle |
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| ZHANG Qian,GENG Xi-lin,YANG Tong-qun,LI Hui-ping,KANG Ying-xin. Effects of recombinant human erythropoietin postconditioning on apoptosis and ultrastructure from ischemia/reperfusion injury in rabbits skeletal muscle[J]. Journal of Lanzhou University (Medical Sciences), 2011, 37(1): 35-38,43 |
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| Authors: | ZHANG Qian GENG Xi-lin YANG Tong-qun LI Hui-ping KANG Ying-xin |
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| Affiliation: | ZHANG Qian~1,GENG Xi-lin~1,YANG Tong-qun~1,LI Hui-ping~2,KANG Ying-xin~1 (1.Department of Emergency Center,Second Hospital of Lanzhou University,Lanzhou 730030,China,2.Department of Medical Education,Emergency Medical Rescue Center in Gansu Province,China) |
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| Abstract: | Objective To observe the effects of recombinant human erythropoietin(rhEPO) postconditioning on apoptosis and ultrastructure from ischemia/reperfusion(I/R) injury in rabbits skeletal muscle.Methods 24 adult rabbits were randomly divided into 3 groups,namely,blank control group,I/R group and rhEPO postconditioning group(5 000 IU/kg).With 25%urethane (4 mL/kg) ear vein injection of anesthesia,I/R models of the left hind limbs of rabbits were set up in I/R group and rhEPO postconditioning group.After 4 hours t... |
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| Keywords: | recombinant human erythropoietin postconditioning rabbit ischemia/reperfusion injury skeletal muscle cell apoptosis ultrastructure |
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