| 神经酰胺诱导人结肠癌细胞凋亡作用 |
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| 引用本文： | 张晓峰,李百祥,任锐.神经酰胺诱导人结肠癌细胞凋亡作用[J].卫生毒理学杂志,2006,20(2):68-71. |
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| 作者姓名： | 张晓峰 李百祥 任锐 |
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| 作者单位： | 哈尔滨医科大学公共卫生学院卫生毒理学教研室 黑龙江哈尔滨150086 |
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| 摘 要： | 目的探讨外源性神经酰胺诱导人结肠癌HT-29细胞的凋亡作用。方法采用光镜、电镜、荧光显微镜和琼脂糖凝胶电泳方法检测C2-神经酰胺诱导HT-29细胞凋亡。MTT法检测C2-神经酰胺对HT-29细胞线粒体功能的影响。结果C2-神经酰胺使HT-29细胞发生核染色质断裂、DNA Ladder、凋亡小体等典型凋亡表现,12和24 h凋亡细胞率分别为64.1%和81.3%,呈现时间-剂量依赖关系。同时C2-神经酰胺处理细胞6 h后,细胞线粒体功能即出现损伤。结论外源性神经酰胺能诱导人结肠癌HT-29细胞凋亡,参与其凋亡调控。
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| 关 键 词： | 神经酰胺 凋亡 线粒体功能 人结肠癌细胞 |
| 文章编号： | 1002-3127（2006）02-0068-04 |
| 收稿时间： | 2005-11-02 |
| 修稿时间： | 2005年11月2日 |
Apoptosis study of human colon cancer cells induced by ceramide |
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| ZHANG Xiao-feng,LI Bai-xiang,REN Rui.Apoptosis study of human colon cancer cells induced by ceramide[J].Journal of Health Toxicology,2006,20(2):68-71. |
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| Authors: | ZHANG Xiao-feng LI Bai-xiang REN Rui |
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| Institution: | Department of Toxicology, Public Health College, Harbin Medical University, Harbin Heilongjiang 150086,China |
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| Abstract: | Objective To investigate the effect of exogenous ceramide on apoptosis of human colon cancer HT-29 cells.Methods Light microscopy,transmission electron microscopy,fluorescence microscopy and agarose gel electrophoresis were applied to detect the apoptosis of HT-29 cells induced by C_2-ceramide.MTT test was used to detect the mitochondrial function of HT-29 cells resulted by C_2-ceramide.Results Under the contribution of C_2-ceramide,HT-29 cells displayed typical apoptosis appearance,such as nuclear chromatin breakage,DNA ladder and apoptotic body etc.The rate of apoptotic cell was 64.1% and 81.3%,respectively for 12 h and 24 h,which appeared time-dependent relationship.Also ceramide damaged mitochondrial function of cells after 6 h exposed.Conclusion Exogenous ceramide induces apoptosis of HT-29 cells and participates in regulation of apoptosis in vitro. |
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| Keywords: | Ceramide Apoptosis Mitochondrial function Human colon cancer cells |
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