| 肾癌G250抗原蛋白多糖(PG)区蛋白cDNA的克隆和序列测定 |
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| 引用本文： | 郑典宝,郑骏年,郝林,武艺,刘俊杰,裴东生,胡书群,陈家存,孙晓青.肾癌G250抗原蛋白多糖(PG)区蛋白cDNA的克隆和序列测定[J].徐州医学院学报,2006,26(1):34-36. |
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| 作者姓名： | 郑典宝 郑骏年 郝林 武艺 刘俊杰 裴东生 胡书群 陈家存 孙晓青 |
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| 作者单位： | 1. 徐州医学院附属医院泌尿外科,江苏,徐州,221002
2. 徐州医学院生物化学与分子生物学研究中心,江苏,徐州,221002 |
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| 基金项目： | 卫生部科研项目;江苏省青年科技创新人才基金 |
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| 摘 要： | 目的 克隆肾癌G250抗原蛋白多糖（PG）区cDNA，为制备G250杂交瘤及抗G250的单克隆抗体奠定基础。方法 从肾癌细胞786-0中提取总RNA，以RT-PCR方法扩增出全长360bp的G250抗原PG区cDNA，将其与表达载体pCA13连接，转化大肠杆菌JM109，构建G250抗原PG区cDNA克隆。结果 酶切及序列分析表明，与GenBank报道的G250抗原PG区cDNA序列完全一致。结论 G250抗原PG区cDNA已成功地得到克隆。
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| 关 键 词： | G250抗原 PG区蛋白 cDNA克隆 序列分析 |
| 文章编号： | 1000-2065（2006）01-0034-03 |
| 收稿时间： | 09 2 2005 12:00AM |
| 修稿时间： | 2005-09-022005-12-29 |
Cloning and sequencing of the cDNA of PG region protein of G250 antigen |
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| ZHENG Dian-bao, ZHENG Jun-nian, HAO Lin, et al.Cloning and sequencing of the cDNA of PG region protein of G250 antigen[J].Acta Academiae Medicinae Xuzhou,2006,26(1):34-36. |
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| Authors: | ZHENG Dian-bao ZHENG Jun-nian HAO Lin |
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| Institution: | Department of Urology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002, China |
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| Abstract: | Objective To obtain the cDNA of proteoglycan(PG) region protein of G250 antigen to get ready for preparing the hybridoma and monoclonal antibody of G250 antigen.Methods The total RNA was extracted from human renal carcinoma cell line 786-0 and the intact cDNA of PG region protein was amplified by RT-PCR.The cDNA was cloned into pCA13 vector and transformed into E.coli JM109.Results The sequence determined was completely consistent with the published PG region protein cDNA.Conclusion PG region protein cDNA has been cloned successfully. |
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| Keywords: | G250 antigen proteoglycan(PG) protein cDNA cloning DNA sequencing |
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