HPLC 法同时测定夏枯草中熊果酸和齐墩果酸含量

目的：建立高效液相色谱（HPLC）法同时测定夏枯草中熊果酸和齐墩果酸含量。方法：色谱柱 Thermo BDS HyPersil C18（250 mm ×4.6 mm，5μm）；流动相：乙腈-甲醇-0.5%乙酸铵（55∶15∶30）；流速0.8 ml／ min；检测波长210 nm；柱温：30℃；进样量：20μl。结果：熊果酸进样量在31.81~795.2μg／ ml 范围内，峰面积与进样量之间线性关系良好（r =0.9998），平均回收率为97.88%；齐墩果酸进样量在33.81~845.2μg／ ml 范围内，峰面积与进样量之间线性关系良好（r =0.9999），平均回收率为98.39%。结论：本方法可使齐墩果酸、熊果酸得到基线分离，同时测定夏枯草中熊果酸和齐墩果酸的含量。实验结果准确，灵敏度及重复性好，分离度高，为夏枯草的质量控制与评价提供了有效的手段。

Simultaneous determination of ursolic acid and oleanolic acid in Prunella Vulgaris Linn by HPLC

Objective:To establish a quantitative HPLC method for simultaneous determination of ursolic acid and oleanolic acid in Prunella Vulgaris Linn. Method:The seParation was Performed on a Thermo BDS HyPersil C18 column(250 mm × 4. 6 mm,5μm). The mobile Phase consisted of acetonitrile - methanol - water - 0. 5% ammonium acetate water solution(55∶ 15∶ 30)and the flow rate was 0. 8 ml/ min. The detection wavelength was at 210 nm. The column temPerature was 30 ℃ . Results:The linear ranges of ursolic acid and oleanolic acid were 31. 81 ~ 795. 2 μg/ ml and 33. 81 ~ 845. 2 μg/ ml,resPectively. The average recover-ies of ursolic acid and oleanolic acid were 97. 88% and 98. 39% ,resPectively. Conclusion:The method can be used for quality control of Prunella Vulgaris Linn,with a good sensitivity,resolution,accuracy and reProducibility.