Low efficient generation of ssDNA aptamer using chemical modified spacer primer |
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Authors: | Shuo Ren Soyoun Kim |
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Institution: | 1. Department of Medical Bioengineering, Dongguk University, 3-26 Pil-Dong, Jung-Gu, 100-715, Seoul, Korea 2. Nanobio Lab, National Research Laboratory, MOST, Dongguk University, 3-26 Pil-Dong, Jung-Gu, 100-715, Seoul, Korea
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Abstract: | Aptamers are oligonucleotides (ssDNA or RNA) with an appropriate size of 100 bps that bind with high affinity and specificity to a wide range of target molecules, including virtually any class of protein, drugs or small organic/inorganic molecules. The in vitro selection process referred to as SELEX provides a powerful tool to identify specific aptamers with high affinity and even discriminate between closely related targets. Aptamers have various applications such as analytical tools, disease diagnosis and prediction, pharmaceutical research, drug development, therapy and even for environmental monitoring. Nowadays, with the development of SELEX methods, generation of aptamer becomes more efficient, less time consuming and even automatically. The whole SELEX process includes binding, separation, and nucleic acid amplification. As amplification of nucleotides is an important process in successive SELEX, we will compare several methods for generation of aptamer in this report. |
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